FastQCFastQC Report
Wed 6 Sept 2023
SRR13150124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16092191
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG12181427.569770952880189No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG11910357.401322790662874No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG7259534.5112129230879745No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6551614.071297687182559No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2419791.5037044986602508No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC2285241.4200925156804316No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2222561.3811419464260648No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2195911.3645811188793373No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1942051.2068275848826304No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1741191.0820092801533365No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1429840.8885303436927887No Hit
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1227590.7628482659695003No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1219500.7578209828605689No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1142980.7102699688314662No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1006790.6256388580026175No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG941200.5848799582356436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT906260.5631675636959566No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG810930.5039276503740231No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC794010.4934132337852564No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG778500.4837750185788871No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA750300.46625099093094285No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG746930.4641568074850715No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC744970.46293882542159737No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG654570.4067625098409533No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC641570.398684057379135No Hit
TGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC635900.39516060926694196No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA621400.38615002767491385No Hit
CGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC618620.38442248168692505No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA607240.3773507286857334Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCA562500.3495484238286757No Hit
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT533060.33125383609975795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG530950.3299426411232628No Hit
TGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT524170.32572941745471456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC512900.3187260205897382No Hit
CCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA500010.3107159242641353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA494870.3075218284446164No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA493100.3064219160709688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG465990.28957523559097703No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA459620.2856167938846861No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG457600.2843615266560035No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC452860.2814159986045406No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT431960.2684283327236173No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG429530.26691828353267744No Hit
GCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGC427200.26547037628375153No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA387250.24064467044916382No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA375990.23364748777838892No Hit
GCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCA374190.23252893282213716No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG367670.2284772782028252No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA361000.22433241067049228No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC356500.22153602327986288No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA342770.2130039346413425No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG341970.21250679910523063No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA340600.21165545449963896No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA330460.20535426157942072No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC323550.20106025338625425No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG321480.19977391518656473No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG307620.19116104202342615No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG296810.18444349809171418No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA291400.18108161902875747No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA290280.18038562927820084No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG285590.17747117219774486No Hit
AAGAGGTGCACAATCGACCGATCCTACTGTAGGCACCATCAATAGATCGG281950.17520920550843574No Hit
GACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT278190.17287266848870983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG274800.17076605665443567No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC273640.17004521012707344No Hit
TTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGC268040.16656526137429017No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG267360.16614269616859506No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC254150.15793374562854742No Hit
GTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGA248990.1547272214206257No Hit
AAGAGGTACACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG247460.1537764497078117No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA244590.15199297597201028No Hit
CGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA240530.14947001312624242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA240330.14934572924221443No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA239570.14887345048290812No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA238940.14848195624822003No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC236880.1472018322427319No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC233930.14536864495331928No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC218910.13603492526281846No Hit
AAGAGGTGTACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG215100.13366731727208556No Hit
CCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA207170.12873946127037642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA207080.12868353352256384No Hit
AAGAGGTGTACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG206760.12848467930811908No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT202160.12562614997547566No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC200760.12475616278727986No Hit
GGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAG199920.12423417047436239No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT199020.12367489299623649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA197360.12264333675880432No Hit
TTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGG196860.12233262704873438No Hit
GTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAG189480.11774655172810215No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG180420.11211649178163496No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA174610.10850604495062231No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC171110.10633107698013278No Hit
CAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC170820.10615086534829221No Hit
TTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCAC164360.10213649589418868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)