FastQCFastQC Report
Wed 6 Sept 2023
SRR13150125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15544648
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG174710811.239289561268933No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG14612689.400457314954961No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG13658068.786342411870631No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA8217675.286494747259636No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT7453584.794949361349321No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA5131893.301387075474466No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1435140.9232373740466815No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1433370.9220987184785401No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1382040.8890777070024358No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA1049830.675364279718653No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1006870.647727758132574No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG839240.5398899994390353No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC766350.4929992625114445No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG659910.4245255344476119No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG629040.40466660936934695No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA550650.3542376771735198No Hit
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA545870.35116266383130706No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC485600.3123904767737423No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG481580.3098043776867768No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG469670.30214257666046856No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA461740.2970411423919024No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG453010.2914250615388653No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC423060.27215798003274183No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT423010.2721258146212124No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG410890.2643289188664806No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG409270.26328675953292735No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA404860.26044977023603233No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA391230.25168147905311206No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC380950.24506827044266294No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG371800.23918200013277885No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG357990.23029791346835257No Hit
CAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA330880.21285782733710018No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC319840.2057557044714039No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA317830.20446265492792118No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT315070.2026871242114971No Hit
TTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGG311880.20063497095592No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC307430.19777224932980147No Hit
AAGAGGTACACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG294800.1896472663774696No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA293970.18911332054608118No Hit
AAGAGGTGCACAATCGACCGATCCTACTGTAGGCACCATCAATAGATCGG286580.1843592727220327No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG280040.18015203689398435No Hit
AAGAGGTGTACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG276860.17810631672071314No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG271670.1747675470039592No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA265510.17080476830353442No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA264010.16983980595765177No Hit
AGAGGTACACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG263330.16940235636085166No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC255790.16455181230221488No Hit
GTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGA248240.15969483516127222No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC247860.15945037803364864No Hit
GCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGC240340.1546127001396236No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG239600.1541366520489882No Hit
GGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAG230910.1485463035251747No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG229410.14758134117929206No Hit
AAGAGGTGTACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG227070.14607599991971512No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA226060.14542625860682082No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG212680.1368187944815476No Hit
CACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCA212620.1367801959877123No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC206810.13304257516799353No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT203700.1310418865708635No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG190390.12247945402173147No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC187920.12089048269217804No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG186450.11994481959321304No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT183660.11814998962987133No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG178690.11495274772384681No Hit
AGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA168360.10830737370186833No Hit
GCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCA165960.1067634339484561No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC162480.10452472130600834No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA160150.10302581312873729No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA157800.10151403878685449No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT157750.10148187337532506No Hit
CACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC157040.10102512453160728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)