FastQCFastQC Report
Wed 6 Sept 2023
SRR13150126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939401
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG13448289.647674243678047No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA8385046.015351735702272No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA6586974.725432606465658No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6320814.534491833616093No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4275863.067463228871886No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3620442.5972708583388915No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1476761.0594142459923492No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1394551.0004375367348999No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA916070.6571803192977949No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA897330.6437364130639474No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA883880.6340875049078508No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA879680.6310744629557612No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG691400.4960040965892293No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC617510.44299607996068124No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG591250.42415739385071133No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT517230.37105611639983666No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT485580.3483506931180185No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC483800.34707373724308527No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC441330.3166061439799314No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA440580.3160681007742011No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG423040.3034850636695221No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC401130.28776702815278793No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA379750.2724292098347698No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC376500.2700976892766052No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG347550.24932922153541606No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG319730.2293714055575272No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA302710.21716141174215448No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA301760.21647989034822945No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA292280.2096790242277986No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG288810.2071896776626198No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG283330.20325837530608382No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA270580.19411164080866888No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA259100.1858759928062906No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG248400.1781999097378718No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG240420.17247513002890152No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCCTGTAGGCACCATCAATAGA237670.1705023049412238No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA235470.16892404487108162No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG226300.16234556994235264No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA223890.16061665777460596No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG212300.15230209676872056No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT211380.15164209710302473No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG209030.1499562283917365No Hit
AAGAGGTGCACAATCGACCGATCCTACTGTAGGCACCATCAATAGATCGG208070.1492675330884017No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA192580.1381551474127188No Hit
AAGAGGTGTACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG189500.13594558331451978No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCCTGTAGGCACCATCA180840.12973297776568735No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG179650.12887928254592862No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG178060.1277386309497804No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG170490.12230798152660935No Hit
TGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAG169880.12187037305261539No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA166750.11962493940736764No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA161240.11567211532260246No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC157500.11298907320336075No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG157470.11296755147513153No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA156520.1122860300812065No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA153650.11022711808061193No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA152500.10940211849849216No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCG152030.1090649447562345No Hit
AGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA149810.10747233686727285No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA146270.10493277293622588No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA144350.10355538232955636No Hit
CTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA141680.1016399485171565No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA140990.10114494876788466No Hit
TTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGG140210.10058538383392515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)