FastQCFastQC Report
Wed 6 Sept 2023
SRR13150127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14571690
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA153774710.552976353463462No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT10679887.3291979173314825No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG9590836.5818240711955855No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3750892.574094013803478No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT3480262.3883708753068453No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3171732.1766383995267535No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2768571.89996493200171No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA2225491.5272696578090805No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2019401.385837881536047No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1926531.3221047112586117No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1453520.997495829241495No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT972590.6674517506205526No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA783010.5373501632274637No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA736890.5056997506809437No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG666270.45723591429683175No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT588580.40392020417672897No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA551570.37852164024900337No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA521330.35776907139803277No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT504650.34632221794452117No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC465460.31942760242634866No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC450960.309476800563284No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA434470.29816033692728844No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG423710.2907761556826971No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC422540.28997322891167737No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA410550.2817449451642191No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA386290.26509622425401586No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA369120.2533131023237524No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA357820.24555833949253658No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG356710.24479658845336402No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG354200.24307407033775766No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC338290.23215563877628467No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT300660.2063315922861384No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA298160.20461593679250656No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC275290.1889211203367626No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG271060.18601823124153752No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC261840.17969089378102335No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA245080.16818913935171556No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA244560.16783228300904013No Hit
TTTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA240470.16502547062145845No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA217680.1493855551415107No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG216210.1483767497112552No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCCTGTAGGCACCATCAATAGA210270.14430035225838594No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG199380.1368269569281257No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA194340.13336819545296394No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG191580.1314741117879944No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG189700.13018393885678325No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG186220.12779574640964775No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG183520.12594283847652538No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT179670.12330072901633236No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCG178650.12260074157493057No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG176350.12102233852078928No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA173210.1188674752207877No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT172640.11847630576823966No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG163890.11247151154052824No Hit
AAGAGGTGTACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG163760.11238229745485939No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA163440.11216269355167452No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG155980.10704317755867714No Hit
TTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT148630.10199915040739954No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCCTGTAGGCACCATCA148370.10182072223606184No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT147690.10135406394179398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)