FastQCFastQC Report
Wed 6 Sept 2023
SRR13150129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12759409
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT203124115.919553954262303No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA183751214.401231279599235No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG5174004.055046750206063No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3039802.382398745898027No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2265831.7758110896829156No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1746711.3689583898439182No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1648811.2922306981459721No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1625981.2743380198879117No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1490031.167789197759865No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1278001.0016137894788073No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1080360.8467163330213806No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1061430.8318802226654854No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC1047840.8212292591294785No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1029700.8070122997076119No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG983990.7711877564235147No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT932370.7307313371645976No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA863520.6767711576609857No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC843020.6607045827906293No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG758190.5942203122417347No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG729730.5719152039095228No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG624960.4898032502916083No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG624260.4892546355399376No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA603400.47290591594015047No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC453670.35555722055778605No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT432060.33862069943835177No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT382580.299841473848828No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG382190.29953581705861143No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA365530.28647878596884857No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG357730.2803656501645178No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA355370.278516034716028No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA311410.2440630283111075No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG294040.23044954511607865No Hit
TTTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA279130.21876405090549256No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT277860.21776870699888998No Hit
TTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT264930.20763500880017247No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA258570.20265045191356434No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC254890.19976630579049545No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA247660.1940998991410966No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA245640.19251675371484683No Hit
TTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGG213270.1671472401268742No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC197060.15444288994889968No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG192820.15111985202449424No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCCTGTAGGCACCATCAATAGA177150.13883871894066566No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG176820.1385800862720209No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG173410.13590754869602503No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA163670.12827396629420687No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG154960.12144763131270422No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG151460.11870455755435068No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG147060.11525612197242051No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA141720.11107097515253253No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT129730.10167398819177283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)