FastQCFastQC Report
Sat 23 Sept 2023
SRR13362463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13362463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330060145
Total Bases25 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62107101.881690381006165No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC38560071.16827404290209TruSeq Adapter, Index 3 (100% over 50bp)
AGTGATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG8164910.24737642892328002No Hit
GGACATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG6960700.21089186638998783No Hit
GCTCATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG5370470.16271185968242244No Hit
AGTTATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG4605220.13952669141559035No Hit
GCGGATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG4467910.13536654054369393No Hit
AGTAATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG4053360.12280670845612093No Hit
AGTCATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATTCATCTCCGGGG3588920.10873533367683637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)