FastQCFastQC Report
Wed 6 Sept 2023
SRR13435518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92695629
Total Bases9.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1409842415.209373033112488No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG72274657.7969857672576985No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA61225696.605024493657624No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA57255946.176768054510963No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA52035655.613603420286409No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA51262205.5301636714715No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA40115924.32770352095027No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA34608853.7336010741132144No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC28901113.1178503573237526No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA28621793.087717329152597No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG28431213.0671575679150953No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC26048812.810144370453541No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA19210772.0724569440054177No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA16297781.7582037228529948No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG15647381.688038602122221No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA14857591.6028360948928886No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG10087541.0882433302221834No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG9060610.9774581711938111No Hit
GCATTTGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA7355300.7934894103798573No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5155550.556180486137054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG4902270.5288566519139754No Hit
GCATTTGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA4590040.495173294525031No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG4536870.4894373174812806Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4297290.46359143859954816No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA3912850.4221180698822379No Hit
GCATTGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA3683870.39741571849089025No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC3656340.39444578341444775No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG3600150.3883840089158897Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA3506540.3782853666163698No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA3461730.3734512659706964No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3083720.3326715653442515Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2936890.3168315520033852No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG2636620.2844384388394408Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2575850.2778825741610751No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGA2429420.2620857128009779RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG2336250.2520345376803042Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG2123700.22910465389905277No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA2016190.21750648026780203No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1988600.21453007239424418No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1968490.21236060656107097RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1947840.2101328855538593No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1818220.1961494861855892No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG1777390.19174474774856967Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA1580080.17045895443462605RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGGAATTCTCGGGTGCCA1445690.15596096769568282No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAA1343450.14493132141106677No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC1254720.13535913327693153No Hit
GCATTTGTGGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1201000.12956382225962348No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1163900.12556147604327708No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT966730.10429078592260267No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA940210.10142980959760249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)