FastQCFastQC Report
Sat 9 Sept 2023
SRR13435519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77203008
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1809255323.435036365422445No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG56611797.332847704586848No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA46243595.989868943966536No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG44622535.779895260039609No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA44015785.701303762672045No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC41104385.324194103939577No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG29137823.774181959335056No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG20936912.7119293072104136Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC19236582.4916878886377067No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA18595552.408656149770745No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG15012171.9445058410159355No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA12151541.573972350921871No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA11628761.5062573727697242No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA10536831.3648211738071137No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG9697171.2560611627982163Illumina Small RNA Adapter 2 (100% over 21bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC6657260.8623057795882771No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG6374280.8256517673508266Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG6268340.8119295040939337Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5680090.7357342864153688No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG5641950.7307940643970764Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG4920850.6373909679788643No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA4790340.6204861862377176No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA3924970.5083959941042712RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3039690.3937268869109349No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3039540.3937074576161592No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2935540.3802364799050317No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2670380.34589066788693No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA2240480.290206309059875No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1902240.24639454462706945No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1856880.24051912588690846No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1780230.2305907562565438No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1515510.19630193683645072RNA PCR Primer, Index 1 (100% over 23bp)
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1511190.1957423731469116No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA1508510.19539523641358636No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGTGGAATTCTCGGGTGCCAAG1473360.19084230500448895No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1393470.18049426260696994Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACT1373410.17789591825230436RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAA1352270.17515768297525403No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG1335240.17295181037505689Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1300330.16842996583760053No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1223850.1585236160746483No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA1145720.14840354406916373RNA PCR Primer, Index 1 (100% over 22bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGAA1032240.1337046349282142RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1011280.13098971480489466No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGTGGAATTCTCGGGTGCCAAG997370.12918797153603134No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA970390.12569328904904847RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG940340.12180095366232363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA936690.12132817415611578No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG911130.1180174223263425No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA845510.10951775350514839No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA844740.10941801645863332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)