FastQCFastQC Report
Wed 6 Sept 2023
SRR13435520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72980844
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1217437716.681606203403184No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA56925577.800070111548724No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG56213497.702499302419687No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA30374954.162044220809504No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC30038974.116007482730674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA24795293.397506611460947No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG24420133.346101341332802No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA24109083.303480568133742No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG18802412.5763486648633442No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC18076422.476871876132318No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC14075461.9286513047177145No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA13835541.8957769246954719No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG13111531.796571440034319Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA12829921.7579846020964078No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG8776551.2025826941656086No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA8503101.165113957848994No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG8169091.1193471536174615No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA8116051.1120794930790332No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA7550721.0346167002398603No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG5941200.8140766363293908No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG5533920.7582702112899653Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG4998670.6849290479567487Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4696140.6434757043916893No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4266950.5846671216901794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG4232690.5799727391478235Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3969490.5439084809707052No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG3687760.5053052003618923Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2849690.39047095700893786No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA2599060.35612906860874344RNA PCR Primer, Index 1 (100% over 22bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGAA2489220.34107854384364206RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA2347660.32168167307026485RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2272570.3113926717537002No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA2267900.3107527778111198RNA PCR Primer, Index 1 (100% over 22bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG2154320.2951897898029242No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAA2000040.27404999591399626No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1589390.21778180586675594No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1584330.21708847324374597No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1583370.21695693187653461No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1552690.2127530890160711No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1533710.21015240656849626No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1446420.19819173371028706Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA1406040.19265877495195863No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1243390.17037210476765657No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1227220.16815645486368996No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1215040.16648752376719567No Hit
AAGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG1173330.16077232540637648Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGA1099140.1506066441215725RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1094730.15000237596594526No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1038400.14228391220030287No Hit
GCATTTGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1011550.13860486458610974No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT940040.1288064029514375RNA PCR Primer, Index 1 (100% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGTGGAATTCTCGGGTGCCAAG917770.12575491727664864No Hit
GCATTTGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG902910.1237187665300226No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA901110.12347212646650126No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA872420.11954095789848634No Hit
GCATTGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG839670.11505347896497334No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA836250.1145848628442828RNA PCR Primer, Index 1 (100% over 22bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA833580.11421901341672616No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA781450.10707604313263354No Hit
GCATGGGTGGTTCAGTGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACT775250.10622650513606008RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC765210.10485080167064113No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA761980.10440821977887786No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA746610.10230218768092075No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA744640.10203225383362242No Hit
GCATTGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG743070.10181712888932883Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG740650.10148553502615015Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)