FastQCFastQC Report
Thu 14 Sept 2023
SRR13435521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100161827
Total Bases10.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1868310318.652917543127483No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG90405369.025929608891818No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA59723715.962721706344274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA42713244.2644230121720925No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA40294874.022976737435111No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG38152343.8090698964586576No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG35164593.5107776139107365No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC32537123.248455122528865No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG27422202.7377895173577453Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC24630812.4591015097997366No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA22018142.1982566272478237No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG16265761.623948013647954No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA15732151.5706732266375294No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC15594701.55695043382146No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA15503081.5478032364565395No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG9626540.9610986828345294Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA8917780.8903371940290186No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA8013000.8000053753013112No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA6906180.6895021992759777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG6463120.6452677825056047Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG6179250.6169266461163893No Hit
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGAA5960660.5951029627285054RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5585340.5576316015082272No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG5483910.5475049891012871Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG5341310.5332680283477657No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG5044180.5036030343176547Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA4263920.425703097448492RNA PCR Primer, Index 1 (100% over 22bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTGGAATTCTCGGGTGCCA3968790.3962377802872945No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA3714680.3708678357075096RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2987010.29821840210642325No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2970110.2965311325641055No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA2897700.28930183152509786No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA2838040.28334547052541287No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2743810.2739376948465606No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTTGGAATTCTCGGGTGCC2671120.26668043904590516No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2365680.23618578762545933No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2280230.22765459340113675No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA2271480.22678100709964089No Hit
CACTGCACTCCAGCCTGGGCAACATAGCGATGGAATTCTCGGGTGCCAAG2102180.20987836014612632No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1925520.1922409023150107No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1777630.1774757962432135No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1733990.17311884696352434No Hit
GCATGGGTGGTTCAGTGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACT1619860.16172428643898437RNA PCR Primer, Index 1 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1507550.1505114318651556RNA PCR Primer, Index 1 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG1491960.14895495067197606No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1443880.14415471874329927No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1436630.1434308900934884No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1427630.14253234418337835No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGTGGAATTCTCGGGTGCCAAG1426770.14244648312974562No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGTGGAATTCTCGGGTGCCAAG1413290.14110066103326968No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTGGAATTCTCGGGTGC1346150.13439750854384874No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1296030.12939360620888035Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA1206780.1204830259336224RNA PCR Primer, Index 1 (100% over 22bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG1196930.11949961735422418Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1169760.11678700709003641No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1132220.11303907226053295No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA1113250.11114513715888988No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT1111620.1109824005107255RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC1041560.10398771979269109No Hit
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1038000.10363229496602533No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC1029010.10273474744025984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)