FastQCFastQC Report
Sat 9 Sept 2023
SRR13435522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88975072
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1303533214.650543918638245No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG68079007.651468941772814No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA44621655.0150732106179134No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG29160603.2773898738738874No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA28898113.247888352369077No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA24953212.804516977519276No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG24506772.754341126017858No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA20833762.3415277483563033No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG18804862.1134975872792774No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC16469031.8509712473174507No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC16040141.8027678583952143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC14673371.649155170113265No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA12582311.4141387826019405No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG12472411.4017870083881472Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA12174571.3683124639674356No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG10937811.2293117335156525Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA9532181.0713315298019652No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG8504570.9558373833066413Illumina Small RNA Adapter 2 (100% over 21bp)
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA6795000.7636970498883103No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA5538440.6224709770395016RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG5404010.60736225085606No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA5193550.5837084346501007No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG4821160.5418551389314976Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA4754920.5344103570941757No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTGGAATTCTCGGGTGCCA4570400.5136719642103802No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4529380.5090616841535122No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG4479620.5034691064931086Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGAA3695720.4153657779563246RNA PCR Primer, Index 1 (100% over 23bp)
CACTGCACTCCAGCCTGGGCAACATAGCGATGGAATTCTCGGGTGCCAAG3524640.3961379205177828No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3480330.391157873971712No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTTGGAATTCTCGGGTGCC3238300.3639558729438286No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG3230260.3630522490614Illumina Small RNA Adapter 2 (100% over 21bp)
AAATGGATTTTTGGAAGTAGGAGTGGAATTCTCGGGTGCCAAGGAACTCC2829450.3180048002658542RNA PCR Primer, Index 1 (100% over 27bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG2771380.31147825314488087No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2634510.29609529284786645No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2528390.28416835672805074No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA2508190.28189805791896405No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA2302520.2587825947474367RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC2288480.2572046246840913No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA2243760.2521784978156578No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAAC2205710.24790201911834361RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1999040.22467416491666342No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1902260.2137969610184749No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1868310.20998128554478718No Hit
AAATGGATTTTTGGAACTAGGAGTGGAATTCTCGGGTGCCAAGGAACTCC1852070.20815605521510563RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTGGAATTCTCGGGTGCCAA1838820.20666687406558099No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1803610.20270958589390073No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACCTGGAATTCTCGGGTGCCA1736490.19516589995004444No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1727830.19419259362892113RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1661110.186693864097126RNA PCR Primer, Index 1 (100% over 23bp)
CATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAG1627050.18286582561012146No Hit
GCATGGGTGGTTCAGTGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACT1625540.18269611515459072RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAA1601710.18001783690604936No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1594820.17924346270829655Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1491850.1676705583334622RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1464350.16457980500426006No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1430300.16075289042755705No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGG1426120.16028309592151835Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGA1390290.15625612531114333RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1330940.14958571767157436No Hit
AATGGATTTTTGGAAGTAGGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1296950.1457655465566805RNA PCR Primer, Index 1 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC1289190.14489339216269473No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1194210.1342184921187813No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT1180200.1326438937863405RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1160470.13042641876142566No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1136960.12778410564253323No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAAG1115700.1253946723414846No Hit
AAATGGATTTTTGGAAGTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTC1068390.12007745270495539RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA1039350.11681361718931793RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGACCCTGGAATTCTCGGGTGCC1028740.11562114835939666No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA991270.11140985645957106No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAA964630.10841575941630034No Hit
TAAATGGATTTTTGGAAGTAGGAGTGGAATTCTCGGGTGCCAAGGAACTC959950.1078897693951852RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG952130.10701087153939028No Hit
TGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACT932830.10484172465744113RNA PCR Primer, Index 1 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA928460.10435057585567338No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA905140.10172961703250995No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA904740.10168466062044884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)