FastQCFastQC Report
Thu 14 Sept 2023
SRR13435523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13435523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101714548
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1353177213.303674121424597No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1074545510.564324584129302No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA48458624.764177883383997No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG42594134.187614342050657No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA32376453.1830697414100486No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA32318723.177394053798479No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG30636813.0120381599690145No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA29930682.9426154457275864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC28668932.8185673105483398No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA28643842.8161006034259723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA23950512.3546789000133983No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG20767332.0417266171206894No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC19286521.8961417397243903No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA14788421.4539139474915623No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG11579741.1384546485916647Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG11265261.107536750790064No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC11160381.097225541424025No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG9098230.8944865979250087No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG7316700.7193366282274587Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG6724650.6611296154017221No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGCCA6702060.6589086941624123No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA6421140.6312902260549789No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA6215550.6110777781758417No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA5060120.4974824250312748No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4886180.48038162643164867No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG4727800.4648105991681741Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGAA4279800.4207657689242251RNA PCR Primer, Index 1 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAAG4111620.40423126099916407No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTGGAATTCTCGGGTGCCAA3969920.3903001171474507No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT3933060.3866762500876472No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG3898490.3832775228967247Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA3882260.38168188094391375No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG3533400.34738393567850295Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA3502080.34430473013555546RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3088500.30364387992954556No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA3028400.2977351873008372RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA2983200.2932913785351531RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2639900.25954006107366273No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG2388360.23481006866392407Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTTGGAATTCTCGGGTGCCA2276850.22384703513601611No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA2077710.2042687148351679No Hit
AAGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG2046390.20118950929222043Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1970240.19370287129428132No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1893540.1861621604020695No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA1770800.1740950566874662No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1745070.1715654283790358No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1617190.15899298888886573No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA1611460.15842964764489736No Hit
CATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAG1579060.15524426260046892No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTGGAATTCTCGGGTGCCA1546710.15206379327370162No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA1504240.14788838269231655No Hit
CACTGCACTCCAGCCTGGGCAACATAGCGATGGAATTCTCGGGTGCCAAG1380430.13571608261976448No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA1373710.13505541016610526No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGA1372410.1349276015069152RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1365340.13423251902962788No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1343150.1320509235316073Illumina Small RNA Adapter 2 (100% over 21bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCTTGGAATTCTCGGGTGCC1311610.12895008883095072No Hit
GGTTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGGAAC1298140.12762579449303557RNA PCR Primer, Index 1 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGGCGCTTGGAATTCTCGGGTGCCAA1296800.1274940532597166No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACCTGGAATTCTCGGGTGCCA1217000.11964856787251318No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1184250.11642877280445664No Hit
GGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCCAAGGAA1163240.1143631882432393RNA PCR Primer, Index 1 (100% over 23bp)
TGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGA1143330.11240574947056738RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1142980.11237133944693928No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAG1142280.11230251939968311No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT1133640.11145308338783554RNA PCR Primer, Index 1 (100% over 25bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGG1131250.11121811208363232Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1052900.10351518250860241No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAAG1052330.10345914332726525No Hit
GCGTTTGTGGTATAGTGGTGAGCATAGCTGCTGGAATTCTCGGGTGCCAA1024200.10069356057109942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)