FastQCFastQC Report
Wed 6 Sept 2023
SRR13531014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40444731
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA28007756.924944067497939No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT26485416.548543987101805No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT19611584.848982677125482No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT18064124.46637165172393No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12628923.1225130413155675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC7195601.7791192627786299No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT5461861.3504503219467574No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA4803471.187662739059879No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC4428351.094913945650918No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC3901170.9645681658755499No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3388040.8376962625860955No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3082440.7621363583800322No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2795190.6911135099402689No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC2621170.6480868917140282No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2584400.6389954726117476No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT2239450.5537062417351719No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1998600.4941558394837637No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1840550.45507781965467886No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT1830120.45249899177225333No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1828910.45219981806777254No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC1779890.440079574271368No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT1677330.4147215121791761No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC1614710.3992386548448054No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC1482710.36660152344689845No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1431690.35398677765961656No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1386590.34283575776533165No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT1272030.31451068372787544No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1263950.31251289568473084No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG1225770.3030728526788817No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT1199450.2965652064789354No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT1189330.29406302640509585No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC1186350.29332621843868856No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1186020.2932446256101938No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1177800.2912122224276878No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT1128600.27904747345210434No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1079220.26683821929734186No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1055040.26085969022763433No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1052330.2601896400299955No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT994500.2458911149637761No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT951360.23522470702055107No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC935160.23121924089444432No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC886840.2192720727948469No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA853970.21114493257477718No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT846310.2092509899497168No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATCT809050.20003841785967125No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC800230.19785766407990202No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC760270.18797751430217202No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG749500.18531462108129734No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGC724740.17919268643423542No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCG723600.17891082029943528No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA707460.17492018923305486No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA702020.1735751438178684No Hit
TTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCGT687470.16997764183423547No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC667310.1649930617661915No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCTCG647780.1601642498252739No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCG647450.16008265699677915No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT647060.15998622910855803No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT641160.1585274482354698No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTC637240.15755822433335012No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT631780.1562082338982549No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATC628520.15540219565312474No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATC627230.15508324186900885No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA624060.15429945621346822No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT619710.1532239143833099No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT617600.15270221478293428No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATC610490.1509442602053652No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA606430.14994042116388412No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAATA596600.1475099438787218No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATCT585130.14467397496104006No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAAT579130.1431904689884079No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA574350.142008609230211No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA570180.14097757257923166No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA544380.13459849689691347No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT541720.13394080924904656No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT541150.13379987618164652No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC532550.1316735176208738No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC512900.12681503556050355No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA511220.12639965388816654No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCGT508860.12581614153893125No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC496950.12287138218325644No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA490370.12124447063326987No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT482080.11919475988108315No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA444610.10993026508199547No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT442670.10945059815084442No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA438450.10840719895009315No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATA435930.10778412644158765No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGTAGGCACCATCAATATCT433740.10724264676157692No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCG431230.10662204676302582No Hit
GGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTA429120.10610034716265018No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT418430.1034572340214106No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATAT413470.1022308690840347No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATCT408850.10108856948510797No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAT407050.10064351769331831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)