FastQCFastQC Report
Wed 6 Sept 2023
SRR13531016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62796656
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT31435895.005981528697961No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA23539813.748576994290906No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT22430213.571879687351505No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT16958432.700530741636943No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11219911.786705011808272No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC9554441.5214886601605029No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC7613941.2124753904093237No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT6977771.111169040593499No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT6640101.0573970690413834No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT4047440.6445311355432685No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3848790.6128972854860297No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3760900.5989013172930737No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC3609060.5747216858171555No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC3354780.5342290837907038No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT3336130.5312591804251487No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT3219590.5127008673837664No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2725310.433989669768403No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC2542210.4048320662170291No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC2513920.4003270492619862No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT1964320.3128064653633786No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATC1904390.30326296355653076No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1898000.302245393448976No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1886310.30038382935549945No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGTAGGCACCATCAATATCT1685020.2683295747467827No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATATCT1619920.2579627806932904No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT1616300.257386316876491No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1576120.25098788699831404No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCTCG1573370.2505499655905244No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC1528650.2434285672791239No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC1513610.24103353528888544No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC1512230.2408137783642492No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1459590.23243116639841457No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1433920.22834336911188394No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1417680.2257572441437009No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1400820.22307238780357985No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATATCTCGT1372180.2185116353966364No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT1352750.2154175215954174No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCG1318100.20989971185726833No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC1288060.2051160176427229No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCTCGT1254410.1997574520528609No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT1247990.19873510462085753No Hit
AACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCGT1225320.19512503977918824No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1211470.19291950832541147No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATCTCG1186920.18901006448496238No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATATC1165310.18556879844047747No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATCT1156340.1841403784303419No Hit
AAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAATATCTCG1140590.18163228309481957No Hit
AGTACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATATCTCG1120410.17841873618238527No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATC1115230.1775938514942579No Hit
ATGATTAAGAGGGACGGCCGGGGGCCTGTAGGCACCATCAATATCTCGTA1109590.1766957144979185No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1081780.17226713473405336No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1080010.17198527259158514No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATATCTCGTATG1057310.16837043042546726No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG1050930.16735445275939534No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT1031640.16428263313893657No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATATCT1022480.16282395674062644No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG989970.1576469294798118No Hit
GAGTGCCTAGTGGGCCACTTTTGGTAAGCCTGTAGGCACCATCAATATCT988200.15736506733734357No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT973730.15506080451162876No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA973220.15497958999600234No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC968380.15420884831829262No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT948310.1510128182621699No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA923610.14707948779947772No Hit
ATGGTGACCACGGGTGACGGGGAATCCTGTAGGCACCATCAATATCTCGT903440.14386753332852628No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC886770.1412129333765798No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACCTGTAGGCACCATCAATAT880090.14014918246602176No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTCTGTAGGCACCATCAATATCTCG878560.13990553891914245No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAT861970.13726367849905893No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCTCGTATGCCG858570.13672224839488267No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT849260.13523968537432948No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAATA840540.1338510764012657No Hit
AGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATATCTCGTA811560.12923618098390463No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCG808130.12868997355527978No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCCTGTAGGCACCATCAATATCT795420.1266659804305503No Hit
GGAGGTGGAGCACGAGCGTACGCGTTCTGTAGGCACCATCAATATCTCGT792290.12616754624641158No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC790340.12585702015725167No Hit
TTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCGT785500.12508627847954198No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATATCT785440.12507672383064472No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG770700.12272946508489241No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATATC753840.12004460874477138No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT751850.11972771288967998No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCG734460.11695845715096677No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT730900.11639154798306457No Hit
ATGAATGGATGAACGAGATTCCCCTGTAGGCACCATCAATATCTCGTATG727990.11592814751154902No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCCTGTAGGCACCATCAATATCT725810.11558099526828307No Hit
GCCGCCTGGATACCGCAGCTAGGAATCTGTAGGCACCATCAATATCTCGT717230.11421468047597949No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATATCT713500.1136206998028685No Hit
AGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATATCTCGTA706760.11254739424341322No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCCTGTAGGCACCATCAATATCTCG699750.11143109276392042No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA690000.10987846231812089No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT689280.10976380653135416No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCT688670.10966666760089899No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA680390.10834812605308156No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT680330.10833857140418433No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATA679670.10823347026631483No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTCG678840.1081012976232365No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATATCTCGT677650.1079117970867748No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCTC673330.10722386236617441No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC670830.10682575199545657No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATCTCGT665230.10593398476504863No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA663070.10559001740474844No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCCTGTAGGCACCATCAATATCT653620.10408516020343503No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCT651650.10377144923130939No Hit
AGCTGGCGCTCTCGCTCCCGACGTACGCCTGTAGGCACCATCAATATCTC640860.10205320487129124No Hit
AAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATATCTCGTA634950.10111207195491428No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATATC634260.10100219349259616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)