FastQCFastQC Report
Wed 6 Sept 2023
SRR13531017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68355896
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT39274565.745599472501977No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT37314225.458815140101448No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA16109282.356677469343683No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC14590742.1345254548342107No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT14266132.0870372323113138No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10064521.4723704301966871No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC9476311.3863193308152963No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT9211721.347611623728844No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT6759190.9888232611273211No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA6277410.9183421427172865No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT5835590.853706898963039No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC5259090.7693688924800284No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5080900.7433009143790611No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC4810790.7037856690518693No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT4304110.6296618509689347No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3960020.5793238376979214No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC3210120.46961859734820827No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT3053180.4466593488877682No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC2549320.3729480775147765No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2540860.37171043738494774No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT2487460.36389838266475216No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC2321230.3395800707520534No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT2239990.3276952144698681No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCTCG2182740.31931993108538875No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT2119980.31013857239176557No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2055070.3006426834051008No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC2051670.3001452866626165No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATC1942470.28417007363929514No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1908730.2792341424359356No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1874080.2741650844573817No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCTCGTA1872870.2739880697343211No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1808090.2645111988583984No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1704180.2493098766491189No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCT1681230.2459524486373494No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATC1575760.2305229090991653No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATCTCG1568290.2294300991972953No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGTAGGCACCATCAATATCT1564570.2288858886437536No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1563530.22873374375781716No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1541870.22556503392187266No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATATCT1535840.22468288616976068No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATCT1477300.21611888460945639No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1467840.2147349513200734No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1454330.2127585307344958No Hit
AAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAATATCTCG1451690.21237231679327268No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC1439280.21055681868320472No Hit
AAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATATCTCGTA1417750.20740712695800226No Hit
TTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCGT1373570.2009438951688966No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT1371760.2006791045501035No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC1352190.1978161474176273No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG1331120.1947337505458198No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG1322310.19344490781014706No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1285760.18809789282844014No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC1271640.1860322334155345No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATATC1255230.18363156266724964No Hit
GCCGCCTGGATACCGCAGCTAGGAATCTGTAGGCACCATCAATATCTCGT1247140.18244805100645597No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC1191210.1742658745925882No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1173280.17164283824178092No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCGT1133580.1658349998074782No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATATCTCGT1123750.1643969380490602No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT1094360.1600973820897615No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCTC1088090.15918012397935652No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAACTGTAGGCACCATCAATATCTCG1057580.1547167196813571No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATATC1053180.15407302977931853No Hit
AGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATATCTCGTA1044930.15286611121299618No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1033930.1512568864578997No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC1025010.14995195147467602No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1021270.14940481505794323No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1007020.14732013753429551No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCGT1005740.1471328822900661No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCG1003590.1468183519970245No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT969880.1418868095884516No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATATCT950170.13900337141363783No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT928810.13587854952555958No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT927120.13563131408591292No Hit
GAGTGCCTAGTGGGCCACTTTTGGTAAGCCTGTAGGCACCATCAATATCT924630.13526704411862292No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT922500.13495543968877244No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC920390.134646761122113No Hit
AACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCGT910610.13321601402167269No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT905680.13249478874507037No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA880500.12881112698749497No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATC845040.12362357154970217No Hit
CCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTCG835500.12222793480755485No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT829480.12134724998703841No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACCTGTAGGCACCATCAATAT811680.11874323174697324No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAT806650.11800737715441548No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT802140.11734759500482593No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC791040.11572374093377404No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG768210.11238386810115107No Hit
ATGATTAAGAGGGACGGCCGGGGGCCTGTAGGCACCATCAATATCTCGTA750840.1098427559196942No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATA718910.1051716153351278No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAATA718200.10506774719184428No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATCTCGT714020.10445624178490763No Hit
ATTGATCATCGACACTTCGAACGCCTGTAGGCACCATCAATATCTCGTAT695540.10175274419634554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)