FastQCFastQC Report
Wed 6 Sept 2023
SRR13531018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72382892
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT32366484.471564910669775No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA29043864.012531027359338No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT26465113.6562659032744924No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT24457013.378838469178601No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA22841513.155650371084924No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC11407321.5759690839653104No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT7249631.0015667790670757No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT6435190.8890484784719571No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC5960370.823450104756798No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5377880.7429766691278376No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA5037330.6959282588487898No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC4837470.6683167619221404No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC4099390.5663479154715179No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC3820160.5277711202807426No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3555090.49115058845673093No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT3465010.4787056587902014No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3092930.4273012468194832No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT3064800.4234149693825441No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2933580.40528637623376523No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT2815920.38903115393620913No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT2700600.3730992124492622No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT2683260.3707036187501323No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC2648900.36595664069349426No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT2543090.3513385455778694No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2295220.3170942658660281No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC2253620.3113470514551975No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC2194400.30316556017131785No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC2114920.2921850649460096No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2080060.28736900979308755No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT2010590.2777714380353855No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT1960480.27084853144580073No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1798440.24846202608207477No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1733750.23952483136484792No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC1641160.22673313467497266No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC1638780.22640432769666072No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1627640.2248652899914527No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1625250.22453510147121503No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC1614440.22304165464955447No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT1596950.2206253378215394No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1552000.21441530686560575No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1501730.20747029560521016No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCTCG1453240.20077119880758565No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1443450.1994186692623445No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1377480.19030463717863055No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC1365250.18861501140352335No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATCT1250550.1727687255159686No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT1233930.1704726028354877No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1219680.16850390559139306No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG1195930.165222743517902No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1170970.1617744148714036No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1167610.16131021678437496No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT1135250.156839547112873No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1131740.1563546258969592No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1119660.1546857232507372No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATA1090860.1507068825047775No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC1077300.14883351165355482No Hit
AACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCGT1045860.14448994383921548No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG1019480.14084543623927046No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCT1011680.1397678335372397No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC1007720.13922074293467027No Hit
CGCGCCGGGCCGTACCCATATCCGCAGCCTGTAGGCACCATCAATATCTC1006560.13906048407129132No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGC1004490.13877450489267548No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1002140.13844984254014056No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTCGT999170.13803952458821347No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGTAGGCACCATCAATATCT995870.13758361575273892No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATC991280.1369494880088516No Hit
AAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAATATCTCG989970.13676850601658744No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC985380.13613437827270014No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC974330.13460777444482322No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA961370.1328172961091414No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT953190.13168719481393476No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCGTA908790.12555314866391357No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCG905820.12514283071198645No Hit
AGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATATCTCGTA886300.12244606087305823No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATATC880520.12164752964001493No Hit
GAGTGCCTAGTGGGCCACTTTTGGTAAGCCTGTAGGCACCATCAATATCT861110.11896595676226919No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATATCTCG844050.11660904623705833No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA841530.11626089767178685No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATATCTCGTA833020.1150852054930328No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT817920.11299907718525531No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT815380.11264816553613248No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGTA810500.11197397307640042No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG792300.10945956677166202No Hit
AGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCTCG789360.10905339344551195No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCGT788900.10898984251693067No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA779680.10771606086145329No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTCTGTAGGCACCATCAATATCTCG779120.10763869451361517No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG730720.1009520315933218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)