FastQCFastQC Report
Wed 6 Sept 2023
SRR13531020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75058180
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA829553711.052142484669893No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT38981445.193496564931364No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA35109674.6776607159939125No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT33023794.399758960315851No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT28197613.7567670838807974No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT16824622.241543826402399No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA12783081.7030895233537502No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT8722091.1620438971475193No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT7158930.9537841178669666No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC6525830.8694362160126984No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC6416400.8548568590392146No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC6075430.8094294319419949No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5756690.7669637073534157No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC5730650.7634943986118502No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA5502130.7330486830349471No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT5154660.6867552610521599No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC5045830.6722558420681131No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT4438810.5913825781547062No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4011790.5344907110723974No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA3722330.49592596036834363No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3705670.49370634886164305No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT3663640.48810669270158163No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA3574110.47617861237775816No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC3520920.46909210961416864No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAT3267880.43537959486894034No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC3065310.4083911973351872No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT2962120.39464319545184817No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT2904130.3869171887727627No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT2731230.3638817248166689No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2722450.36271196557124086No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA2718940.36224432833303444No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2566810.3419760511112846No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC2324620.3097090816750419No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC2300060.306436953307421No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAAT2248670.29959026451214243No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2113630.28159888768952296No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA2030930.2705807681454573No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT2025610.26987198463911594No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC1975000.2631292152301055No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT1869030.24901083399570836No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT1849900.24646214443249226No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1829820.24378688638600085No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1793970.23901059151714044No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1780480.23721331905463203No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1759940.23447677521623894No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG1738870.23166961948717651No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1721810.22939671598751796No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG1699390.22640969978222225No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1660150.22118175527304287No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1608830.21434439257653196No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1446300.19269052353787422No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT1439060.19172593846533448No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT1407670.18754384931795573No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1390950.18531624401231153No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT1295760.1726340819881324No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1279510.17046909477421382No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT1258460.1676646036447993No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1223860.16305484625393263No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC1216090.16201964929072354No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATCT1184730.15784155704281666No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATCA1163910.15506770880935294No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGC1143930.15240577376110107No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1134490.15114808272729235No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1115920.14867400195421737No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC1113500.1483515853968215No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT1112450.14821169391530678No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATA1096520.14608934029575457No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATC1094980.1458841661228663No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1090750.14532060329733548No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA1083150.14430805543113356No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATCT1083130.14430539083148566No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT1074650.14317560058077614No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1064270.14179267336351614No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1062980.14162080668622662No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG1054600.14050433943375656No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT1034840.1378717149816316No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTC992280.1322014469309008No Hit
AGCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATA953150.12698815771978483No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATC950030.12657248017471245No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAT949450.12649520678492338No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT943630.12571980828738452No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAA929170.12379330274195298No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT908490.12103810670602458No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC905510.1206410813584875No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA899330.11981772006728647No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT895270.11927680633876281No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATAT893670.11906363836693082No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCTGTAGGCACCATCAATATCTC872490.11624182733980494No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT864060.11511869858821518No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAT857770.11428068199895068No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAAT856320.11408749852447794No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC850710.11334007832324204No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA846310.11275386640070409No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG834670.11120306940562641No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA834100.11112712831566125No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAA830190.11060619908449686No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCCTGTAGGCACCATCAATATCT829540.11051959959594011No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCTGTAGGCACCATCAATATCTCG826860.11016254324312154No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATC823600.10972821350051387No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATCT822460.10957633132058359No Hit
CGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTCG816890.1088342403186435No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT798080.10632818434979371No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA796980.10618163136915924No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC796800.10615764997232813No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAT796190.10607637968306718No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC786750.10481868864925849No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT785070.1045948622788349No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGCTGTAGGCACCATCAATA768710.10241521976685286No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCTGTAGGCACCATCAATATCTC766010.1020554988143864No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTCGT765850.1020341820172032No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT765450.10198089002424518No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATA754650.10054200621437931No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)