FastQCFastQC Report
Wed 6 Sept 2023
SRR13531021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13531021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74221863
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA766103810.321807740126383No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT61115638.234181618426904No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA30316724.084607792720051No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT27709043.7332719605812104No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT25934973.4942493965693098No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT16940072.2823558066711422No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA12747561.7174939411046581No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT12219381.6463316206439065No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC11582751.5605577025195394No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC11060491.4901929907094895No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA8723651.1753477543402542No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8436681.1366839444598689No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC8369181.1275895890675771No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT7147320.9629669360360841No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC6982600.9407740142550719No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT6296800.8483753634693864No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC5812330.7831021433671099No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT5211840.7021974104853713No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC4635960.6246084122140669No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT4627820.623511700319352No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT4410740.5942642533777412No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4336260.5842294742722909No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAT4299360.5792578933245047No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC3919880.5281301009649946No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3834280.5165971110156585No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC3717300.5008362562928392No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC3596210.48452165637502254No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT3494800.47085856629602524No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA3491560.4704220372371952No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT3210960.43261646504346035No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2705070.36445730283002997No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2674930.3603965047333829No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC2596500.34982953742349476No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC2450100.3301048910615461No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA2321290.31275016634923325No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC2119320.2855385077035859No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC2033700.27400282313042995No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC2030920.2736282704194585No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT1910390.2573891199686001No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGC1884260.25386859394785066No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA1870760.25204972286939226No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1808340.2436398019273647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC1774250.2390468156262798No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT1719340.23164872592863914No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1709980.23038764198090797No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAAT1650080.22231724364019265No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT1631130.21976408757080107No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1573110.21194698386915994No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1434500.1932718934850773No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1411690.19019867501843762No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1406840.18954522874210258No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATCT1398270.188390582435259No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT1386770.18684117373879444No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1353710.18238696056443637No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATATC1343260.18097901961851862No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT1338720.1803673400113926No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1311100.17664606451605777No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1297990.17487973860208816No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG1281870.1727078718032179No Hit
TAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1217870.1640850755794152No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC1185220.15968610219336588No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATAT1156690.15584222131422382No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1146000.15440194488246678No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTC1140290.1536326297818744No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1130900.15236750389841333No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1102820.1485842520552199No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATC1079760.145477350790831No Hit
TGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1072940.14455848406823202No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATCT1052160.1417587699193161No Hit
GCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCTC1036430.13963944828493458No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1028470.13856698800459913No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAT1013030.13648673841560674No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT1004560.13534556522786284No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAT1002100.13501412649801042No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG992430.13371127588107026No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT978250.13180078759273398No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATATCT965060.13002368318348462No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA961460.12953865089589572No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCG955900.12878954547395285No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT944660.12727516688714752No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG940820.12675779911371934No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTCGT929740.1252649775174735No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT923920.12448084198587146No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT872970.11761628780457856No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT869060.11708948884778062No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT866250.11671089420107927No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATCT857450.1155252597203064No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT828090.11156955195263693No Hit
GCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATATC827560.11149814442140855No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT827430.11148062936657895No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATC813160.10955801527105294No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAAT759070.10227040515002972No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATA756350.1019039363105181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)