FastQCFastQC Report
Wed 6 Sept 2023
SRR13554798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39548813
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6989161.7672237090908391No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6365391.6095021612911617Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTTAGTGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4140371.0469012053534958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC3975501.0052134813755345No Hit
ACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1944520.4916759448633768No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1765520.4464154208623151No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1734540.4385820631329694No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1588470.4016479584355667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1510760.3819988225689605No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1489400.3765979019395601No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1452970.3673865003230312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC1375900.34789918979363554No Hit
TAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1150470.2908987432821309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1100280.2782080969155762No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC1051010.26575007447126164No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG1047860.2649535903896787No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1035460.26181822448122527No Hit
TGTTAGTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG987460.24968132419043776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA982160.24834120811666333No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCA931160.23544575155770164No Hit
TACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACA871710.22041369484338252No Hit
ATCCCTCGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG821800.20779384706185747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA795970.20126267759287744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGCCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA724470.1831837532013919Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT698140.17652615768771618No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA649320.16418191868362775No Hit
ACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA624140.15781510307275215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT584000.1476656202045811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA529210.13381185422682598No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC513010.12971565037868518No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA487020.12314402457540255No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG461120.11659515546016515No Hit
ATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTGTAGGCAC447600.11317659521159333No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC445060.11253435090453916No Hit
TTGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT442810.1119654337034085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCT429690.10864801429059325No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGA424930.10744443834509015No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC424130.10724215667357703No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC421630.10661002645009851No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCA420270.1062661476085262No Hit
GCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA412470.10429390131127324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)