FastQCFastQC Report
Wed 6 Sept 2023
SRR13554799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42542885
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC8407401.9762176448541275No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5572371.3098241926940308Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5185981.2190005449794954No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2307360.5423609611807003No Hit
ACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2153830.5062726705064784No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2101970.4940826180452971No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC1703740.4004758962632647No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1492130.35073549901470014No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA1284310.3018859675360521No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC1267880.29802398215353754No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1198000.2815982037889532No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1191270.2800162706407899No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1189700.27964723125852886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1183920.27828860219517315No Hit
TACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1156510.2718456917061455No Hit
ATTCATCTCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1123120.26399714076748676No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA1101830.25899277869848275No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG1060880.24936719735861823No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1036380.24360830253989593No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1008190.2369820476443946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA983210.23111032549861157No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC977400.22974464472731454No Hit
TAACCGTGAGGCTTAACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA888550.20885983637451952No Hit
AGGTTCTGGCGCCGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCA876640.2060603083218263No Hit
AACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT813850.19130108360070078No Hit
TCGACACACTATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT811280.19069698728706339No Hit
TCGACACACTATCATTAACCTGTAGGCACCATCAATAGATCGGAAGAGCA792050.1861768424966948No Hit
TAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT779010.18311170011154626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTGTAGGCAC756780.1778863845270484No Hit
CGGAGGTTCGAATCCTCCCGGATGCACCACTGTAGGCACCATCAATAGAT740460.1740502554069852No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATAGAT724850.17038101670819927No Hit
ATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTGTAGGCAC682730.1604804187586244No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA645380.15170104237171503No Hit
GCGGGAGCGGCGAAATTGGTAGACGCACCAGCTGTAGGCACCATCAATAG632320.14863119884793896No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGA580070.13634947418352095No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG566510.13316210219405666No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT555390.13054826911715084No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCC555300.13052711399332695No Hit
AGGTGTTAGTGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT543180.12767822398504475No Hit
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATC491990.1156456596678857No Hit
TAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG483980.11376285364756059No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGCTGTAGGCACCATCAA443090.10415137572357869No Hit
GCATCCGCAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT432700.10170913420657766No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCT426700.10029879261831914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)