FastQCFastQC Report
Wed 6 Sept 2023
SRR13554802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35664330
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC5707501.6003384894655248No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2613260.7327377242191287No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC2422790.6793314216193043No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1932770.5419336350914206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT1417700.3975120239185764No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA763680.21412991636181022No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT599310.1680418502184115No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA598330.16776706586104378No Hit
ATGTACACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA579890.16259663366730848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA572230.160448829404618Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC544740.15274084778825228No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCA527960.14803586664883372No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA509200.1427757089506518No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC495990.13907172797021564No Hit
AAGGGGGTCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA478960.13429664878044814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACAGTGCTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT459320.1287897459450381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACCA436590.12241643120731556No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA420930.11802548933346006No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGCTGTAGGCACC401170.11248493943388253No Hit
ATTCATCTCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC400230.11222137076457066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)