FastQCFastQC Report
Wed 6 Sept 2023
SRR13554803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32858231
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC2509050.7635986246490263No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1718070.5228735533571481No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1695570.5160259540448175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA1285500.3912261740444883No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1064120.32385188356609945No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT993820.30245693993690653No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA871060.26509643808883077No Hit
ATTCATCTCCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC811860.2470796434537209No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA682820.2078079005531369No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA640400.1948978933162896No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC616390.18759074400566483No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG574450.17482681888748058No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT458300.13947798954849394No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC413660.12589235251283004No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC409320.12457152668991828No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA398040.12113859690133653No Hit
ACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG394990.12021036677233171Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTGTAGGCAC394930.12019210650749883No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA392490.11944952240429499No Hit
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC390450.118828673399977No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC379520.11550226182292041No Hit
TACAGTGCTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT377730.11495749725540612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC377540.11489967308343531No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT375470.1142696939467009No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAAT368670.112200197265641No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC365580.11125979362674758No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCA337020.10256790756629594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)