FastQCFastQC Report
Wed 6 Sept 2023
SRR13554806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17870060
Total Bases893.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG11749326.574863206950621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4308122.4108033213094977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1667780.9332817013485125No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1472950.8242557663488539No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1308110.7320120917333237No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT995030.5568140230083167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA985710.5515985956398579Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC969500.542527557266176No Hit
GTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC818320.45792795323574736Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA754580.42225935447334817No Hit
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT701800.3927239192257888Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA544950.3049514103478108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA460560.2577271704739659No Hit
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT449460.2515156636295569No Hit
TGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA442580.24766564857644577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT429900.24056998129832805No Hit
TTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA385710.21584146891504563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA352710.19737482694518094Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT344580.19282531787805973No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA324620.18165579746234764No Hit
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG308420.17259035504077772No Hit
TAGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA295710.16547789990632378No Hit
TCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT278370.15577451894397668Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTACACGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC272480.15247850314996145No Hit
ACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG246580.1379849871796737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT243710.1363789489235067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA240290.13446513330117527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA238470.13344667001677668Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC222570.12454910615856915No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC221400.12389437976145574No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT219460.1228087650517122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC214710.12015068779847408No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT212190.11874050786622987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCAACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC211650.11843832645217756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACC206890.11577465324682737No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG202550.11334601003018456No Hit
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA201710.11287595005276983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC200910.1124282738838034No Hit
AACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT198700.1111915684670337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT197090.11029062017698878No Hit
ATCTGCCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA183770.10283681196369794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC183440.10265214554399929Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT183190.10251224674119729Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)