FastQCFastQC Report
Wed 6 Sept 2023
SRR13554807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13554807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13132853
Total Bases656.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4188883.1896191939405703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1782211.357062323015418No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1463831.1146321366728158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1018110.7752390131831979No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA841720.6409269943096142No Hit
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC821800.625758926868366No Hit
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT736220.5605941070078223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA676970.5154782437601335No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC448470.34148710870364574No Hit
TAGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA398060.3031024561075952No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA329260.25071475329846454No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC319610.243366768820149No Hit
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC272060.20715986084668733No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG269220.2049973452074732No Hit
GTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC254120.193499462759539Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGA217060.1652801565661323No Hit
TACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT215640.1641988987465252No Hit
TAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT199990.15228221925578547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC190500.14505606664446788No Hit
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA189440.14424893052560628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC189080.14397480882486083No Hit
ATTCCTGTACTTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC181250.13801266183364727No Hit
AGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT180850.13770808216615232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT175880.13392367979752762No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT174670.13300232630335543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA167230.12733714448794942Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT165020.12565434182503984No Hit
ACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC163160.12423804637118835No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA159520.12146637139698435No Hit
ACTTGGTGTTACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT158930.12101711638742929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA155800.11863378048928135Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC154280.11747637775280056No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT153510.11689006189287278No Hit
ATCAGGTAACATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC144980.11039490048354306No Hit
TAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC137350.10458504332607697Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCATTTAAAGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC135280.10300884354679063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)