FastQCFastQC Report
Wed 6 Sept 2023
SRR13566023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13566023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56568967
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA44654177.893757367003007No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT28397345.019950249400877No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT18074183.195069833960376No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11987412.119078822846456No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT11940932.1108623037079677No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT7542531.3333335218937266No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC7166551.2668695187592873No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC4425490.7823176265530887No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCTC3859820.6823211037245916No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3810890.6736714849327194No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3680150.6505598732251908No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3638240.6431512175217907No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3405770.6020562475535394No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAT3205320.5666216248919659No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC2647940.46809056987022585No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT2288230.40450270198499466No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2286080.404122634942229No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC2216960.39190392145573383No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC2207090.3901591485663862No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC2144300.37905942316394076No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC2138380.3780129129810696No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATCT1887280.3336246178934114No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1865050.3296949014465829No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAT1762720.31160547796462323No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1729620.30575421325971885No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1584430.2800881974740673No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1523980.26940212643444594No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1515030.2678199868843283No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCTCG1500170.26519310490502684No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC1426210.25211879863388703No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATATC1340200.23691434916957207No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAT1339640.23681535496308426No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATC1332930.23562919223891785No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1225130.2165728074900148No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1201130.2123301986405373No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1199820.21209862290750334No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCCTGTAGGCACCATCAATATCT1184020.20930557208159734No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC1180690.20871691010373233No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1153760.20395634942388113No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT1121050.1981740271127808No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTC1093710.193340988531751No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT1064780.18822687711444333No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1014040.179257294905173No Hit
TAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCG1011960.1788896021382183No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGCCGT999500.17668698104386454No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATATC985810.17426692624597512No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA983910.17393105304539147No Hit
AAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAATATCTCG981530.1735103276678183No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT979620.17317268671354738No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT967480.17102663373718668No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT964370.1704768623404419No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA926040.16370106245708888No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA907190.16036884675656177No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCGTATGC893330.15791874014598853No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA891120.15752806658109914No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATA891070.15751922781266273No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT835350.14766930426712582No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA822050.14531819186304037No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATATC789930.13964016701948967No Hit
AACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATATCTCGT787270.1391699445386726No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATATCTCGT787230.13916287352392345No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAT784570.13869265104310635No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATCCTGTAGGCACCATCAATATCT783710.1385406242260001No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA772240.1365130107466873No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATATCTCGT771920.13645644262869427No Hit
GAGTGCCTAGTGGGCCACTTTTGGTAAGCCTGTAGGCACCATCAATATCT769970.13611173065967425No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACCTGTAGGCACCATCAATAT764080.13507052373786496No Hit
AGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATATCTCGTA753630.13322322113465496No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT745970.13186912181019675No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGTAGGCACCATCAATATCTCG733860.12972837209489788No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTCTGTAGGCACCATCAATATCTCG733520.1296682684695303No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT726240.12838134378518878No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA725390.1282310847217698No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATATCTCG714820.12636256907431243No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTC697240.12325485809207017No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATATC696120.12305686967909454No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC692190.12236214247999262No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA671870.118770066987435No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG664990.11755385245058479No Hit
GACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCGTA663020.11720560497419019No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAT653090.11545022556271886No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATATCTCGTATG652280.115307037514049No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAT648930.11471484002880943No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATATCT647660.11449033531052459No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAATA642380.11355696136363953No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCTCG627000.11083815619259939No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATATCT621400.1098482141277213No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC617850.1092206615687361No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC617410.10914288040649567No Hit
AGTACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATATCTCG609080.10767034158498952No Hit
AGATACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAT590220.10433635813077513No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT585800.10355501100099637No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATC579830.10249966204968883No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCT578470.10225924754821844No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTCGT575870.10179963158952504No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA574490.1015556815806801No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCGT573320.10134885439926807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)