FastQCFastQC Report
Sat 23 Sept 2023
SRR13577564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151554443
Total Bases22.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA27535421.8168665632587229No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC19151791.2636904349943736TruSeq Adapter, Index 7 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA14813850.9774606211973607No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA12011340.7925429147596814No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA11798060.7784700841795843No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA10518770.6940588340257369No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA9317410.6147896304168396No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGTC8078610.5330500274412938TruSeq Adapter, Index 7 (98% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA7635560.5038163084403933No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA7587590.5006511092518746No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA6710510.442778836909453No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA6122900.4040066314651033No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA5771110.380794510920409No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA4853530.32024993157079534No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4604730.30383338877105703No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4197720.2769776930921121No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA4149280.2737814819457322No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA4058770.26780937065632576No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3770600.24879508151404048No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA3749580.24740812118586322No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA3629230.23946708048671328No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA3192390.21064311522691553No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA3051680.2013586629063722No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA3047980.20111452621682627No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA2912040.19214481227713No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA2733590.18037016572321804No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA2711260.1788967678103637No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA2512390.16577475066171435No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG2401240.15844075254197595No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA2395000.15802901931420119No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1949920.12866135504849566No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1942060.1281427295404332No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA1918400.12658157438512047No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA1869190.12333455641415937No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1856420.12249195492078052No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1845430.12176680296994No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1819360.12004662905197705No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1649260.1088229396217701No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA1540530.10164862009357258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)