FastQCFastQC Report
Wed 6 Sept 2023
SRR13577567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69673487
Total Bases10.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA31072544.4597365996623655No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA17398462.4971421338507143No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA11693031.6782610578971024No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA10328331.4823902813992933No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA7371861.058058139102468No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA6789910.9745328233679476No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA5655340.8116918276244736No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA5208170.7475110295541832No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA4820060.6918069135824937No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4365460.6265597127354915No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA4322700.620422514521198No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA4211800.6045054125107876No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA3902740.5601470757448956No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA3879020.556742624350063No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA3834390.5503370313588581No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA3443530.4942382171858285No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3051080.43791119568911485No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA3049760.43772174055247154No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA2292730.32906778442135387No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA1976870.2837334666485115No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1921910.2758452436864542No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1873840.2689459191270275No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1833650.2631775843227137No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1812670.2601663958630347No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1796760.25788288736000825No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1772250.25436504993642706No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1768820.2538727536343918No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA1763960.25317521426765965No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1727180.2478963052330078No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1638550.23517554102035973No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1605150.2303817519568096No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1579400.22668594152607863No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA1518320.21791933565776606No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1498910.2151334839893976No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA1372980.19705917690038968No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA1371360.19682666377814564No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1290750.18525698304722427No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1277610.18337104327791143No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA1270700.18237927434290752No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA1217440.1747350466325878No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA1131490.1623989337579731No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1130830.16230420618965144No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA1049520.15063405682566167No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA1038390.14903660555987386No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA1022030.14668851007844635No Hit
GGGGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA1003790.14407058455392077No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA983060.14109527774890898No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAA979590.140597240382127No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA928830.13331182921848017No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA918740.13186364563610833No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC850330.12204498965295077TruSeq Adapter, Index 1 (100% over 50bp)
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA839740.12052504276124432No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA827560.11877688854585389No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA811340.11644888679104004No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA808140.11598960161129872No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA794270.11399888741035741No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA787260.11299276581348656No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCAAAAAAAAAACAAAAAAA762270.10940603561294412No Hit
CCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA758520.10886781079293475No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA739400.10612358184398034No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA707810.10158957596022142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)