FastQCFastQC Report
Wed 6 Sept 2023
SRR13577568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48336849
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC32913236.809138510456071TruSeq Adapter, Index 3 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA18841233.897901991915112No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA14171632.9318481227437894No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGCCGTC10267982.12425514124845TruSeq Adapter, Index 3 (98% over 50bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA7736151.600466344010136No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA6314001.3062498136773457No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA5668541.1727160783691133No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA5449161.1273304141111888No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA4200860.8690802331777978No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3982300.8238642117528182No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA3404190.7042639457114799No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA2922870.6046877404027722No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA2848670.5893371328362756No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2825900.584626440999495No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2718210.5623473718777159No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA2477010.5124475532114225No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA2015780.41702759730987016No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1853410.38343624757170247No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1564920.32375300260056256No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA1324560.2740269643972862No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1213770.25110656261437314No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1197100.24765784794950124No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1181050.2443373998168561No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1135820.23498014940940815No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1038310.21480713399419146No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1023300.2117018426252816No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA993510.20553884263328792No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA981940.2031452236367331No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA939870.19444171878063463No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA918350.18998962882334344No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA890220.1841700521273118No Hit
GGGAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAA888700.18385559224185258No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA887620.18363216021797366No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA870910.18017517029295807No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA862030.17833806254106468No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA850930.17604167785119795No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA793320.16412323442928603No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA764990.15826228143253607No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA750560.15527698133570933No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA745370.15420326633206893No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA721830.14933327573752275No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA712920.1474899615405216No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA697710.1443432938708934No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA661180.13678591254469236No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA619730.12821067422082064No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA611290.12646459433050755No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA601570.12445370611559724No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA592540.12258556613816511No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA575410.11904168598164105No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA550620.11391309350760534No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA547680.11330486188704605No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA535730.11083262791912647No Hit
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA516920.1069411868365685No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA497920.10301043826832816No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAAA484690.10027339597581132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)