FastQCFastQC Report
Wed 6 Sept 2023
SRR13577569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58303111
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA28595614.904645654328806No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC16926812.90324301905605TruSeq Adapter, Index 4 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA13158512.2569138720573587No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA11418031.9583912083181978No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA9306641.5962510130891645No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA8323511.4276270780816482No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA5610150.9622385330347124No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA4577490.7851193395151761No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA4047160.6941584986777121No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA3910580.6707326475254468No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA3731660.6400447482124925No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3640180.6243543333390906No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA3535520.6064033186839721No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA3154900.5411203529087838No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA2735680.46921681417651967No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2552760.4378428451270808No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA2414900.41419745165914046No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1994570.34210352857500176No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGCCGTC1933480.33162552852454136TruSeq Adapter, Index 4 (98% over 50bp)
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1907260.32712834140188507No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1878130.32213203854593625No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1867540.3203156689185934No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1751920.30048482318550723No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1702590.2920238681603114No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1694550.29064486798997746No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1683920.2888216376652697No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGCCGTC1624260.2785889075456025TruSeq Adapter, Index 4 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGGCGGC1598710.2742066371038074TruSeq Adapter, Index 4 (97% over 41bp)
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1574700.2700885035105588No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1433310.24583765350017087No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1367720.2345878249961653No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGGC1361350.23349525894081363TruSeq Adapter, Index 4 (98% over 50bp)
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1354100.23225175754343538No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA1353400.23213169533955058No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1323390.22698445714157517No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1304820.2237993783899456No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA1266420.21721310891969384No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA1253780.21504512855240263No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGCCGGC1252450.21481701036502152TruSeq Adapter, Index 4 (97% over 48bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGCCGTC1235580.21192351125139788TruSeq Adapter, Index 4 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGCCGGC1209670.20747949453331915TruSeq Adapter, Index 4 (97% over 41bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1095400.18788019733629652No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGCCGTC1094090.18765550949759782TruSeq Adapter, Index 4 (97% over 41bp)
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1013290.17379690082060972No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1006070.17255854494625508No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA953560.16355216448055407No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGCCGTC933090.16004120260409432TruSeq Adapter, Index 4 (97% over 42bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA920600.15789894985192127No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA897350.15391116950860478No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA891550.1529163683907022No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGGCGTC888500.1523932402166327TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGGCGTC874950.15006917898429126TruSeq Adapter, Index 4 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGGCGGC858170.14719111643973853TruSeq Adapter, Index 4 (97% over 45bp)
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA841720.14436965464844576No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGCCGGC836750.1435172130008637TruSeq Adapter, Index 4 (97% over 48bp)
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA833230.1429134716327573No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA815160.1398141515981883No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA781690.13407346307815377No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA777260.1333136408449971No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGGCGGG775790.13306151021683904TruSeq Adapter, Index 4 (97% over 41bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA769490.13198095038187585No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA754060.12933443637338668No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTCCGTC726650.12463314350412621TruSeq Adapter, Index 4 (98% over 50bp)
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA719930.12348054634683216No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA714960.12262810469925009No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA709610.121710486426702No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA704980.12091636070672113No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA699700.12001074865456149No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGGCGGC698470.1197997822105925TruSeq Adapter, Index 4 (97% over 41bp)
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA681470.11688398583053312No Hit
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA671760.11521855154521686No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA661380.11343820057903942No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA657080.11270067561231852No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA654430.1122461544118975No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA649030.11131996026764335No Hit
GGGGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA624540.10711949830601664No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA619360.10623103799726913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA616810.10579366854026023No Hit
GGGAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAA609740.10458104028102377No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGATGCCGTC599140.1027629554793397TruSeq Adapter, Index 4 (97% over 41bp)
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAA597330.10245250892358042No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGCCGGC585650.10044918529304551TruSeq Adapter, Index 4 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)