FastQCFastQC Report
Wed 6 Sept 2023
SRR13577572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46956988
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC14985423.191307755940394TruSeq Adapter, Index 4 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA6701271.427108144159502No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA4519250.9624233138633168No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGCCGTC2990040.6367614549723675TruSeq Adapter, Index 4 (98% over 50bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA2751600.5859830702940316No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2676400.5699684144988175No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA1949530.41517356266547584No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1555020.33115837838662054No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1467200.3124561566853479No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGGC1389550.29591974681169925TruSeq Adapter, Index 4 (98% over 50bp)
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1312930.27960268661184146No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1286960.27407209338043576No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA1238180.2636838631983806No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA1147910.24445988741867344No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1133930.24148269475887169No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGCCGTC1116670.2378069905165127TruSeq Adapter, Index 4 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGCCGTC1008060.2146773127782387TruSeq Adapter, Index 4 (98% over 50bp)
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA974200.2074664584534255No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTCCGTC946950.20166327533614378TruSeq Adapter, Index 4 (98% over 50bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA938810.1999297740306512No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA919040.19571953805895725No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA891470.18984820747020656No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA842480.17941525550999993No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA808350.17214690175613478No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA756090.16101756782185433No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA751700.16008266969763904No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA747840.15926064082304428No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA738870.15735038201342896No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA680110.14483680256493453No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGCCGGC640100.1363162390228266TruSeq Adapter, Index 4 (97% over 48bp)
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA538700.11472200900108839No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGCCGTC538360.1146496023126526TruSeq Adapter, Index 4 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGGCGTC515170.10971104023963377TruSeq Adapter, Index 4 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGCCGGC491360.10464044244064376TruSeq Adapter, Index 4 (97% over 48bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA483010.10286221935699964No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA477760.10174417490321143No Hit
GGGAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAA475360.10123306886719394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)