FastQCFastQC Report
Wed 6 Sept 2023
SRR13577576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28336768
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA13184404.652753623843058No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA5143941.8152881796540805No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC5114581.8049270827216426TruSeq Adapter, Index 11 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA4824951.7027171200328843No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA2719870.9598377627257985No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA2563780.9047538519565816No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2347170.8283125302081027No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA2344720.8274479291357434No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA2187560.7719864170818634No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2129150.7513736217200211No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2043810.7212572725301629No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2020060.7128759356042298No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1921080.6779460522809094No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1914450.6756063359095857No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA1903280.6716644608164205No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA1746190.6162276516503222No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1586260.5597886110370809No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGCCGTC1423240.502259114377476TruSeq Adapter, Index 11 (98% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1217410.42962203734737847No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1066130.3762355678671611No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA918930.3242889238462198No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA906050.3197435924943875No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA868750.3065804822907115No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA811070.28622530275859265No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA748720.26422208771303773No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA714820.25225883205875843No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA689360.2432740388741581No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA654120.23083789936805776No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA634770.22400931538840277No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA616880.21769596306819464No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA612770.21624555065701212No Hit
CCTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA591550.20875704667518893No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA589680.20809712667302074No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA560250.19771132685280127No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA509080.17965351588438033No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA469010.16551287712134286No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA454560.1604134952864067No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA444990.15703625762825174No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA441220.15570583067200888No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA440420.15542351195450377No Hit
GGGGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA438520.15475300500042913No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA434070.15318260713430693No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA427650.15091699942632836No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA426280.15043352862260084No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA400020.14116641672049543No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA389070.13730217927464416No Hit
GGGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA381030.13446487616371774No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA379180.13381201412948718No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA372860.13158169626119676No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA369920.13054417497436546No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA366820.12945018994403315No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA362160.12780568341456583No Hit
CCCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAAAAAAAAAACAAA353630.12479546008916755No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA349020.12316859847954431No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA342220.12076888938075084No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA339040.11964667247866799No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA329840.11640000722735917No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA314460.11097242988332331No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA312400.11024545918574766No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA304980.1076269530808877No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA301360.10634946088417706No Hit
CCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA298360.10529076569353286No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA297170.104870816601244No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA296440.10461320077152059No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA295170.10416501980748123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)