FastQCFastQC Report
Thu 14 Sept 2023
SRR13577580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51331418
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC33968646.617514443103832TruSeq Adapter, Index 12 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA12480752.4314056549148906No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGTC9707641.891169264016825TruSeq Adapter, Index 12 (98% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA6733731.3118145304304667No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA6216571.211065316761754No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA4813620.9377531709722103No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA4426230.8622847707031978No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA3243180.631811885656461No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA2907530.5664230822534456No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2780030.5415844931460885No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA2631650.5126782197990323No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2263960.4410476250626858No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA2258410.43996641588977725No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA2032950.3960439978494263No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2029670.3954050129688605No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1992180.38810149370897956No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1846490.3597192658889727No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1771240.3450596280040423No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1426340.2778688093128462No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGGC1166650.22727796064390815TruSeq Adapter, Index 12 (98% over 50bp)
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1153610.2247376061187322No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1137170.22153488921736No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1135800.22126799614224568No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1120620.21831074294499328No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1027470.2001639619618535No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA999980.19480856733784366No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA988110.1924961433950646No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA974510.18984669389027983No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA943790.18386205500888364No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA905880.1764767145142961No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA888020.17299736391463022No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA887670.17292917955237475No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA876480.1707492280848349No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA826050.16092483554613668No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA768300.14967441577398077No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA764090.14885425530227903No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTCCGTC732540.14270792207610553TruSeq Adapter, Index 12 (98% over 50bp)
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA717700.13981690511647274No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA716030.13949156830228224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA676680.13182569786012927No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA662630.1290885827467303No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA651010.12682486191984801No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA642300.12512804536200423No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA638920.12446957923507977No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA625180.1217928559853928No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA622130.1211986779714521No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGGC621390.12105451674839762TruSeq Adapter, Index 12 (97% over 48bp)
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA615030.11981550947998357No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGCCGTC595090.11593094895605652TruSeq Adapter, Index 12 (98% over 50bp)
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA594700.11585497209525752No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA591820.11529391220012662No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA558630.10882808653367028No Hit
GGAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT550600.10726374245106574TruSeq Adapter, Index 12 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGCCGTC536310.10447987234640586TruSeq Adapter, Index 12 (98% over 50bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA534530.10413310616122079No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA519160.10113883859588683No Hit
CCTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA515350.10039660310961993No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA515290.10038491436180469No Hit
GGGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA513670.10006931817079356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)