FastQCFastQC Report
Wed 6 Sept 2023
SRR13577581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27006182
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA5361901.9854342979692574No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3749411.3883524890708356No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA3592571.330276897341505No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2582260.9561736642373216No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA2163590.8011461968226385No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2026540.750398556893381No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1609630.5960227921147832No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1565050.579515460571213No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1518460.5622638549943861No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA1460100.5406539880387388No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1298750.4809084083044393No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA1216260.4503635500938266No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1151350.4263283125322936No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG1136220.42072589157549184No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA1124530.416397253043766No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA1123360.41596401890500484No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1095270.4055626967188476No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1053840.3902217647796345No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA909120.3366340343851641No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA824120.3051597593469525No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA789660.29239971796087283No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA782430.28972255315468143No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAACAAAAA712800.2639395676145558No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA702670.26018857460117834No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA700550.2594035691531665No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA629050.23292814956220026No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA610730.22614451757749393No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA607500.2249484951260419No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC597690.22131599350104358TruSeq Adapter, Index 12 (100% over 50bp)
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG594050.21996815395823074No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA556430.20603801011190698No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA545760.20208706288063968No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA542250.2007873604643559No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG511810.1895158671447893No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA509320.18859385602896403No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA502570.18609442830534134No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA495640.1835283491757554No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA484410.17937004201482462No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA483150.1789034821730817No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA473600.17536725480114146No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA460550.17053502786880426No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA459680.17021287940664845No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAAAAAAAAAACAAAAAA456500.16903537123463064No Hit
GGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC453500.16792451446857612No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA451860.16731724610313298No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACATAAAAAAAAAACA449170.16632117786957074No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA442960.16402170436383787No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA437580.16202956789671344No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCAAAAAAAAAACAAA429660.1590969060343295No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA408050.15109503446285003No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA402180.14892145805727No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAAAAAACAA399400.14789206412072614No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA392380.14529265928815854No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC386990.14329681996514723No Hit
CCCTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA381600.14130098064213595No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA380770.14099364360352754No Hit
GGGCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAAAACAAAAAAAAA380720.14097512932409328No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAAA373080.13814614742654108No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA368560.13647245656568557No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA358930.13290660634665055No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA344880.1277040938256285No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA341600.12648955709474224No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA336900.12474921482792348No Hit
CCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA336350.12454555775414682No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT334700.12393458653281683No Hit
CCCTCAAAAAGTAAGTTTAGATTATAGCCAAAAAAAAAACAAAAAAAAAA325880.1206686676406165No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA321470.11903570819451635No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA321280.11896535393266623No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAAA320450.1186580168940578No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA318540.11795077141966977No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG314270.11636965195598549No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTCAAAAAAAAAACAAAAAAAAA311210.11523657805460986No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTTAAAAAAAAAACAAAAAAAA311000.11515881808098605No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCAAAAAAAAAACAAAAAA309590.11463671540094043No Hit
GGGGCAGTTAAAAAGCTCGTAGTTGGATCTTGAAAAAAAAAACAAAAAAA309350.11454784685965605No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA308090.11408128701791316No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA308080.11407758416202632No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAAAAAAAAAACAAAAAA307520.1138702242323628No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA299130.11076352814329696No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAAAAAAAAAACA297470.11014885406608013No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA295340.10936014576218142No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA281630.10428353034131223No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGCAAAAAAAAAACAAAAAAA277450.10273573658060957No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA274630.10169153122051833No Hit
GGGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACG273420.10124348565820968No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGAAAAAAAAAACA270170.10004005749498393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)