FastQCFastQC Report
Wed 6 Sept 2023
SRR13577583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68244930
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA23471173.4392547548953454No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA15179502.22426779542451No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA11348591.6629206008417035No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA8253471.2093894740605637No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC7111501.0420554318100994TruSeq Adapter, Index 4 (100% over 50bp)
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA6776290.9929367646798085No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA6213220.9104295366703432No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA5390560.7898843181464176No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA4687810.6869096356315407No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA4186230.6134126007602323No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA3943640.5778656377843746No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA3589690.5260009791203537No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA3444150.5046748527692826No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2844880.4168632014129108No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2744490.40215295114230465No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA2172720.31837090315720157No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1914270.2804999580188594No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1873840.27457570840793594No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1815270.26599338588229193No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA1782180.261144674043918No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1697750.24877305903896452No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1696840.24863971580013342No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA1665210.24400493926801595No Hit
GGGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1503300.22028009992830236No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA1498770.21961631435478066No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1449330.21237182014839784No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1401190.20531781628320228No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1321570.19365101554064162No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA1222240.1790960881636189No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA1213720.177847643773684No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1206990.17686148993046077No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1197910.17553098816278367No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1192830.17478661052183658No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA1163230.17044929198403455No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1142440.16740291183535538No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA1027410.1505474472609174No Hit
CCTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1011800.14826009785635358No Hit
GGGGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAAA1003110.14698674319103264No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA988070.14478291647452785No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA971030.14228602769465803No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA960580.14075477841357592No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA953460.13971147746799653No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA909200.13322601400572906No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA898350.13163615231197395No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA890740.13052105116087012No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA886870.12995397606825884No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA862450.1263756882745722No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA842810.1234978188123279Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA841130.12325164667910128No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA836590.12258639579526273No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA798790.11704752279766424No Hit
GGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA757760.11103535456773125No Hit
GGGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA737910.10812671358883363No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA730830.10708927388452154No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA705320.10335126726630096No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA693530.10162366640276428No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA682450.10000010257172216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)