FastQCFastQC Report
Thu 14 Sept 2023
SRR13577584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127757146
Total Bases19.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA45592463.5686817863010187No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA41945633.283231608821318No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA17890731.4003701992528856No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA16510501.2923347551924806No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA16107601.26079835878613No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA14759741.1552966281823485No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC11135650.871626390276439No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA9188300.7192004743124114No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA7876880.6165510303431482No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA7707780.6033149801264346No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA7643680.5982976482583604No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA6985710.5467960281454628No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA6813960.5333525531323312No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA6281450.49167112734343643No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA5608400.438989142728658No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA4574240.35804181161028753No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA4408360.34505780208959896No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA4287390.3355890558168856No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA4177420.3269813181330773No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA3996910.31285216718914494No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA3861740.3022719371016632No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA3744640.2931061093052282No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA3610760.2826268520431726No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA3538020.27693323706526757No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA3397600.26594207106035384No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA3342280.2616119805932421No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA3291250.2576176834758034No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA3166990.2478914173614993No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA2834030.22182947011042342No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA2816420.2204510736330945No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA2650130.20743497197409214No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA2593340.20298981945009947No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2552550.19979704305542328No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA2532060.1981932188748174No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA2510210.1964829427232196No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2487490.19470456861959015No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA2422890.1896480999974749Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACA2359440.18468164590965425No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA2282360.17864832390667212No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA2187720.17124051910176516No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA2184800.1710119604581649No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA2126240.16642826382486658No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA2118800.16584590892473441No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA2099870.16436419141673686No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA2086320.16330358538222198No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA2016400.15783070169710897No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1973450.15446885452497508No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC1962340.15359923585018093No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA1903980.1490311939184991No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA1880040.14715732613500931No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA1853440.14507525082002065No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1809050.14160068979624826No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAACAAAA1803410.1411592272106642No Hit
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA1698490.13294677074267142No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA1681760.13163725495245487No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAACAAA1664420.13027999232230814No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA1638710.12826758042951272No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1630000.12758581817411607No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA1595450.1248814684698733No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA1590620.12450340742583589No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA1568090.12273990528874214No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC1563140.12235245142373484No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT1543020.12077758844111937No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1521580.1190994044278353No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA1467250.11484680473372504No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA1444390.11305747233896413No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA1402370.10976841952934673No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1383580.10829766031248068No Hit
GGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1368190.10709303102309439No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA1353980.1059807644732452No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA1338180.10474404304554519No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1333680.10439181225917493No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAACAAAA1316110.10301654672216926No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1304470.10210544308809154No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT1301350.10186122974287481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)