FastQCFastQC Report
Sat 23 Sept 2023
SRR13577585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126008381
Total Bases18.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA49817493.9535060767108816No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA43658663.464742555497162No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA19906831.579802060943867No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA16605371.317798853395315No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC16325961.2956249314876922TruSeq Adapter, Index 12 (100% over 50bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA15504811.2304586311604147No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA13618981.080799538246587No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA11062070.8778836702933276No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA9408500.7466566846851243No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA8947350.7100599126021625No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA8636680.6854052033253248No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA8096770.6425580533409123No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA7707970.6117029628370513No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA7093140.562910176585794No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA6660780.5285981731643707No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA6577500.5219890889638523No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA6244840.495589257670091No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA5839400.4634136200829372No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA5732310.45491497902826006No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA5475040.4344980831076625No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA5450570.4325561487850558No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGTC5121790.4064642335179277TruSeq Adapter, Index 12 (98% over 50bp)
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4792810.3803564462906638No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4097970.32521408238710725No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA3987910.3164797427244145No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA3862420.3065208813372501No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA3792610.3009807736518732No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA3745850.2972699093721393No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA3585900.28457630925358846No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA3518390.27921872910977247No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAACAAAAAA3446140.27348498351073963No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA3330510.2643086097582668No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA3111200.246904211871431No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3096990.24577650910378732No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA3014140.23920154961756074No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA2979220.2364303053778621No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA2611580.20725446825636146No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2533820.20108345015558926No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA2474570.19638138196537896No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA2218780.17608193854978582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA2211230.17548277205466198No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCAAAAAAAAAACAAAAAAA2203860.1748978903236603No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA2157080.17118543884791282No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA2130190.1690514538076638No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA2095040.16626195681380906No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA2090810.16592626485693837No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA2070580.16432081608920918No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA1925350.15279539223664812No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA1924080.15269460528978623No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAACAAAA1885870.14966226730585483No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA1877150.14897024984393697No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA1851310.14691959259440052No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA1850460.14685213676382367No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAACAAA1729860.1372813448019779No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1709210.13564256491796367No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA1639550.13011436120268857No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1634610.12972232378733603No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA1621510.12868271039844564No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC1586190.12587972223847554No Hit
GGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1580520.12542975216862756No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA1567460.12439331317176436No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA1566520.1243187149591264No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA1559120.12373145243410437No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA1551710.12314339631107553No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA1502260.11921905416751605No Hit
GGGAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAA1494630.11861353888833791No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA1478420.11732711651933692No Hit
CCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1477620.11726362867879399No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1472870.11688666962557039No Hit
GGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1401420.11121641186708049No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA1388320.11017679847819012No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1382940.10974984275053895No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA1360460.10796583443128278No Hit
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA1358520.10781187641796619No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT1280480.10161863757300399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA1261380.10010286538004168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)