FastQCFastQC Report
Wed 6 Sept 2023
SRR13577596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15665243
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG4564992.9140882142715565No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG4471562.854446624287922No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA3947232.5197374850808254No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG3939282.5146625558250197No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG3199342.0423175050651943No Hit
GGGCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA2755841.7592066717381913No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAA2739681.7488908406974601No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA2707311.7282272608219356No Hit
GGGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC2434831.5542880503034648No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAA2207281.4090301695288097No Hit
GGGGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC2089071.333570120808212No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1224830.7818774340110779No Hit
CCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGA1100510.7025170308561444No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAAA1048310.669194853855762No Hit
CCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC1046280.6678989914168584No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCG896000.5719668695851063TruSeq Adapter, Index 22 (97% over 36bp)
GGGGGAATGCCTAAAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC841760.5373424465870079No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA802600.5123444302779089No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAAA788890.5035925711461993No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA758480.4841801687978923No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA715250.45658404405217334No Hit
CCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC574450.3667035359745138No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT500570.3195418034689918No Hit
CCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC484610.3093536436045071No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA471810.3011826883247199No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA464270.29636948498022025No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA441380.2817575188587882No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAA430600.27487604245909236No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA402570.2569829271081208No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA373380.2383493189349185No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA366100.23370208811953952No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA339480.216709054561107No Hit
GGGGTCGCGGCGTGGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA327760.20922752363305183No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC320760.2047590324644182No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA316640.2021290062337367No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA296240.18910654625657577No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA291930.18635523240845994No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGA289140.1845742194998188No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC277410.17708630501295128No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA276430.17646071624934256No Hit
CCCCTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCAAAAAAA275890.17611600407347655No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAAGA260130.16605551538523852No Hit
CCCCGCGGCGTGGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA257220.1641978997708494No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA246520.15736749184165225No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAA246280.15721428643015622No Hit
CCCCGGAATGCCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC246100.15709938237153423No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAAA241750.15432253428816903No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAGATCGGAAGAGC233090.148794372356688No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAAACAAAAAAAAAAGATCGGAAGA231800.14797089326989693No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA229230.14633031865512713No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAAAAAAAAAA224740.14346410074838928No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAGATCGGAAGAGCAC222510.14204056713323887No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA220990.14107026619376412No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA220070.1404829787830294No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA218100.13922541769699964No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAACAA216400.13814021269890292No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC206170.13160983203388546No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA205970.1314821608576388No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG203680.13002032588961435No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA203300.12977775065474567No Hit
GGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA202960.1295607096551263No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA190330.12149827487514875No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA186120.11881079661515626No Hit
GGGGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGA184630.1178596463521185No Hit
GGGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA182950.11678720847164642No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA179910.11484660659269698No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAGATCGGAAGAGC175660.1121335940974551No Hit
GGGGGGGAGGTAGTGACAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA174740.1115463066867204No Hit
CCCCGGAATGCCTAAAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG169870.10843751354511387No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAA167550.10695652790065242No Hit
GGGCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC166530.10630540490179437No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAAGATCGGAAGA162370.10364984443586353No Hit
CCCCCCCGAGTGTTACAGCCCTCCGGCCGCAAAAAAAAAACAAAAAAAAA159250.10165817408641538No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAAGATCGGAA158130.10094321549943401No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGAAAAAAAAAACAAAAAAA158110.10093044838180934No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAAACAAAAAAAAAAGATCGGAAGA157620.10061765400000498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)