FastQCFastQC Report
Wed 6 Sept 2023
SRR13577598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758247
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA13787378.227214934831787No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCG8315934.962291103598127TruSeq Adapter, Index 25 (97% over 40bp)
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA7256104.329868153870748No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA4675432.7899278486586336No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA2487571.4843855684905467No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA2098221.252052198538427No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA1933201.1535812785191673No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1921361.1465161004011934No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAA1823221.0879538892104885No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAA1494940.8920622783516676No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA1285620.7671566125024891No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA1214150.7245089537109699No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG1069830.6383901609756677No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA1022790.6103203992637177No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA939500.5606194967767214No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA832110.4965376151813492No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG750110.4476064829453821No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA746340.4453568443047772No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA738190.440493567137422No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA704650.4204795406106617No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA704260.4202468193719785No Hit
CCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAA698610.4168753450166954No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA653730.3900945009343758No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG646650.3858697153705874No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA609450.3636716895269535No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAA609180.3635105748232497No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAA605920.3615652639563076No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA603730.3602584446929324No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA588880.3513971359892237No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA552220.3295213395529974No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC529600.31602350770936843No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAA525820.31376790185751524No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAGAAAAAAAAAACA519630.3100741980948246No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAA496180.2960810877175877No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG484950.2893799094857595No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAGAAAAAAA484530.2891292866133313No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAGAAAAAA449120.2679993915831411No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA414110.24710818500288245No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA413900.24698287356666843No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAAA413150.24653533272304676No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAGAAAAA387130.23100864905499963No Hit
GGGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC381980.22793553526213095No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAAAA374320.22336465144594178No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCAAAAAAAAAAC364810.21768983354881927No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA357600.21338747423880314No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA355670.2122358024678834No Hit
CCCCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC354980.2118240648917515No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA350910.209395409913698No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA345910.20641180428955366No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAA341110.20354754289037513No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAAAA338800.2021691170920204No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC329860.19683443023605032No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA319850.1908612517765134No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAGAAAA315970.18854597381217736No Hit
GGGGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAA309200.18450617179708595No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAAA308750.18423764729091296No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAAAA308600.1841481391221886No Hit
GGGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA306140.1826802051551096No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAAA301700.18003076336086943No Hit
GGGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAG292970.1748213879411134No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAGAAA278390.16612119394110852No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAAAA276620.16506499755016144No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAGAAAAAAAAAA272900.16284519496579802No Hit
CCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGA271890.1622425066297209No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA270580.16146080195619505No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA269730.16095358900009052No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAAAA252200.15049306768184048No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA250190.14929365822093443No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA247930.1479450684788212No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAAAA247210.1475154292689444No Hit
GGGCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA244360.14581477406318213No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAGAAAAAAAA239080.14266408652408574No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAACAAAAAA235910.1407724805583782No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAGAAAAAAAAA228350.13626126885467196No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAAAA223330.13326572880803106No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAGAA222190.13258546672572613No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA218570.13042533625384564No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAAAC212320.12669582922366524No Hit
GGGGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC209930.12526966573532422No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAGAAAAAAA208450.1243865184705775No Hit
CCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAAA207040.1235451416845688No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA204800.12220848636495213No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAGAAAAAAAAAACAAA201840.12044219183545868No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGAAAAAAAAA200880.11986933955562297No Hit
CCCCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACA200710.11976789696440207No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA200140.11942776592324962No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCAAAAAAAAAAC193900.11570422610431748No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGA192750.11501799681076427No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAGAAAAAA187550.11191504696165416No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAACAA185840.11089465383819679No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAAAA184810.11028003107962307No Hit
CCCCTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCAAAAAAA178520.10652665520444948No Hit
CCCCTGAGCGTCGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC173770.10369222986151237No Hit
GGGGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC172340.1028389186530071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)