FastQCFastQC Report
Wed 6 Sept 2023
SRR13577600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16001254
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA7990324.9935586298423855No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCG6243803.9020691753283834TruSeq Adapter, Index 7 (96% over 32bp)
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA3236962.0229414519637023No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAA2661501.6633071383030358No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG2468141.5424666091795056No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA2444171.527486533242957No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAA2235621.3971529981337711No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG2074261.2963109016330845No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1775751.1097567728129307No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA1704141.0650040303091246No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG1695251.059448215746091No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1233560.7709145795698262No Hit
GGGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC1221510.7633839197852869No Hit
GGGCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA1137540.7109067826809073No Hit
GGGGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC1041710.6510177264856867No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA1032370.6451806839638944No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA982430.6139706300518697No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA888620.555343974915966No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA876480.5477570695396748No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAACAAAA863510.5396514548172286No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA855130.5344143652741216No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA789570.49344257643807166No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA690820.43172866326601655No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA687960.42994130335034986No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAACAAA683840.4273665051501589No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA670020.41872968206116845No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA629360.39331917360976837No Hit
CCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGA586090.3662775429975676No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAA536620.33536121606469094No Hit
CCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC535850.3348800037797037No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG503360.3145753451573233No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAA487230.30449488521337137No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA422680.2641542969069799No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAA392750.2454495128944269No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA391980.24496830060943975No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAA389000.24310594657143747No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA374210.23386292099356715No Hit
CCCCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC372830.23300048858670702No Hit
GGGGGAATGCCTAAAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC362600.22660723965759183No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA355120.22193260603200227No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA354180.22134515207370623No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA350980.21934530881142192No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA350700.21917032252597202No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC336530.2103147665801693No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT334980.2093460925000003No Hit
CCCCTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCAAAAAAA309980.1937223170134041No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA301710.18855397208243804No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA300870.1880290132260884No Hit
CCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAA299590.1872290759211747No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA287380.17959842397352108No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA286580.17909846315795No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGAAAAAAA281810.17611744679510744No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA268850.16801808158285594No Hit
CCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC262850.16426837546607284No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC245930.1536942042167445No Hit
GGGGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAA244590.15285676985066296No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAGAAAAAAAAAACA243860.15240055560645435No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAAAAAAAAAA239050.14939454120283321No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA236230.14763217932794517No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA225690.1410451955827962No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAACAA225210.14074521909345356No Hit
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA224750.14045774162450017No Hit
CCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC220900.13805168019956435No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA219380.1371017546499793No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA218810.13674553256888491No Hit
GGGGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC218570.1365955443242136No Hit
CCCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAA217270.13578310799891058No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAA214610.13412073828713675No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAA211640.13226463375932912No Hit
GGGGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAAA204720.12793997270463928No Hit
CCCCCCCGAGTGTTACAGCCCTCCGGCCGCAAAAAAAAAACAAAAAAAAA201330.1258213887486568No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCAAAAAAAAAAC201140.1257026480549587No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA198470.12403402883299022No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA197830.12363406018053334No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA191780.11985310651277707No Hit
GGGGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAA191080.11941564079915236No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA185110.1156846832129532No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA184450.11527221554010704No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTAAAAAAAAAACAAAAA182160.11384107770553484No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAGAAAAAAA179470.11215995946317707No Hit
CCCCGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAA173650.10852274452989748No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAAAA173280.10829151265269585No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAAAAAAAAAACA169500.1059291977991225No Hit
GGGATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGAAAAAAAAA166900.1043043251485165No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATCAAAAAAAAAACAAAAA165220.10325440743581722No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA160620.1003796327462835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)