FastQCFastQC Report
Wed 6 Sept 2023
SRR13577616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14569493
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA8492785.829152737161135No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC7363695.0541841092205475TruSeq Adapter, Index 3 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA4509483.0951523158698797No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA3247412.2289107795309007No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2313221.5877148230209521No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1911351.311885046377386No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA1787891.2271463392720667No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1740901.1948940158727555No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA1513931.0391095970189217No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1480551.0161987105522476No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA1432830.9834453402050435No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1261310.8657198984206246No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1108790.7610354045950672No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA949950.6520130796589833No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA921720.632636976454843No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA833370.571996568446136No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA723600.49665420752801764No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA697220.4785478808356612No Hit
CCCCCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAACAAAAAA693040.4756788722847116No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA550650.3779472628182738No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA503880.34584593986901263No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA497820.3416865638358178No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA492840.3382684627392319No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA466890.320457273290155No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAAAAAAAAAACA457640.3141083907312355No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA450690.30933814924102027No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA424850.2916024600169683No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA399450.27416877169301634No Hit
GGGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAA398380.27343436041322783No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAA394050.2704623970099715No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAAA378500.2597894106541662No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA366020.2512235669422402No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA346550.23786002711281717No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA337980.23197787321768848No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG328930.22576626379517806No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA328200.22526521684728493No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA321780.2208587491685538No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA319710.21943797220671987No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA269860.18522264295675903No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA264570.1815917684987391No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA262700.18030826467331432No Hit
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAA260190.17858548681138048No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA259560.1781530764316919No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA255660.175476250271715No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA250300.17179733021595192No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAAA242090.16616226796635958No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAAA235820.1618587551399352No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA225620.15485782518307262No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA222810.15292913761652516No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA220720.15149463334105037No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA218030.14964830965634837No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA217340.1491747173357371No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA209760.1439720654658333No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA207360.14232478782892447No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA197790.1357562682517504No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAAAAAAAAAACAA197070.13526208496067776No Hit
GGGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT191810.1316518014731192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAA188370.12929070352688318No Hit
GGGGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAG183360.125852011459836No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA179740.1233673676908318No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC177240.1216514534857184No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAAA176000.12080036003998217No Hit
GGGGCAGTTAAAAAGCTCGTAGTTGGATCTTGAAAAAAAAAACAAAAAAA172050.11808921559590302No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA170250.1168537573682214No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA168970.11597520929520334No Hit
CCCCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAACAAAAAAAA167980.11529570726997844No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA167770.11515157047674893No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC160780.1103538743592519No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA159670.10959200845218156No Hit
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAA158330.10867227843824077No Hit
GGGCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA153460.10532967756667991No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA151740.1041491285935619No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA149240.10243321438844852No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA146490.1005457087628238No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAACAAAAAAA146090.10027116249000564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)