FastQCFastQC Report
Wed 6 Sept 2023
SRR13577617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13577617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17331324
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA8318574.7997313996322495No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC5569953.213805246500498TruSeq Adapter, Index 4 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA3855792.2247521308816336No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2938871.6956984936638424No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA2628531.5166354284300496No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2421621.3972504351081314No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2259161.3035126456582313No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA2123871.2254516735132295No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA1838581.06084220686198No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA1670810.9640406007065588No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1667490.9621249940281539No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1636460.944220995464628No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA1555640.8975886666246617No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA1110980.641024309510341No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1060010.6116151310771178No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA866820.5001464400527045No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA836590.4827040334598787No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA753910.434998503288035No Hit
CCCCCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAACAAAAAA730360.4214103896505541No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA701040.40449304392439955No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA647750.37374524877614657No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA630200.3636190749189156No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA630130.3635786856214793No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAAAAAAAAAACA597400.34469380412021605No Hit
GGGCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA589510.3401413533091875No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA542750.31316130262177316No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA452280.2609610206352383No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA443390.25583157986083466No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAA441760.2548910862205334No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA435650.25136567754431227No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAAAAAAAAACAAAAAAAAAAG430010.24811145415087735No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA419440.2420126702380037No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAAA419100.24181649365045627No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA390620.22538381949353667No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA377040.21754829579090437No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA368930.21286890718793325No Hit
CCCCCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAACAAAAAAAAAAG336560.19419174207348497No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA321620.1855715120206627No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA319400.18429059430197023No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA317750.18333856086240152No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAAA312700.18042476154735781No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA305860.17647815019787294No Hit
GGGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAA297320.17155065591065055No Hit
CCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAA296220.17091596695093808No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAACAAAAAAAA289300.1669231964043832No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAAAAAAAAAACAA282040.1627342492702808No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA265290.1530696673837498No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAA236850.13666007282536521No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAAA233160.13453097986051152No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA230690.13310581465097532No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA230180.13281154976965406No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAAAAAAAAAACA228900.1320730026165341No Hit
CCCCTAGACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA228780.13200376382092907No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAAAAAAAAAACAAAAA226730.13082093439601036No Hit
GGGGTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAAAAACAAA224090.1292976808927004No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA221680.1279071350809667No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA220680.12733014511759172No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA214810.12394321403258055No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA200490.11568071775705074No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA197310.1138458896735183No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA197040.11369010238340706No Hit
CCCCGAATACAGACCGTGAAAGCGGGGCCTCAAAAAAAAAACAAAAAAAA196270.11324582011160833No Hit
CCCCCGCCTGGGAATACCGGGTGCTGTAGGCTCAAAAAAAAAACAAAAAA191090.11025701210132591No Hit
GGGCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA189970.10961078334234592No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTCAAAAAAAAAACAAAAAAAAA188720.10888954588812719No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA186580.10765478736650472No Hit
GGGGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAG185730.10716434589763599No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA185090.10679507232107599No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA184570.10649503754012099No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA184570.10649503754012099No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA179960.10383511380896232No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA176660.10193104692982488No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAAA175530.10127904827121113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)