FastQCFastQC Report
Wed 6 Sept 2023
SRR13664942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13664942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11443011
Total Bases869.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG7898246.902239279504318TruSeq Adapter, Index 7 (97% over 36bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAGATCGGAAGAGCAC3350062.9276035826584454No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAGATCGGAAGAGCACA2917532.5496174040206725No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAGATCGGAAGAGCACACGTC2524722.206342369154412No Hit
GGCTCAATGGTCTAGGGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1983081.7330054126488212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG1691731.478395852280488TruSeq Adapter, Index 7 (97% over 36bp)
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG1490151.3022359237441963TruSeq Adapter, Index 7 (97% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAAGATCGGAAGAGCACACGT1359241.187834216011852No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTAGATCGGAAGAGCACA1331321.1634350434514134No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1104590.965296633901689No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1010580.8831416836005838No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCTAGATCGGAAGAGCACACGTC990810.8658647623427086No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGTC961900.840600432875578No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCTG951150.8312060523231166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACGT945420.8261986290146884No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAGATCGGAAGAGCACAC940620.8220039288610315No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAAGATCGGAAGAGCACAC888340.7763166530207828No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTAGATCGGAAGAGCACAC826970.7226856637645459No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA764780.668338079898726No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTC754960.6597564225010357No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACAC747490.653228420386907No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAAGATCGGAAGAGCACACGTC712210.6223973742575271No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG631460.5518302831308998No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAGATCGGAAGAGCA619030.5409677575246584No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCT582340.508904518225142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTAGATCGGAAGAGCACAC562490.49155768529803917No Hit
ATAATTTTGAACGAAAGGGAATACGGTTAGATCGGAAGAGCACACGTCTG427190.3733195747168293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCGTTGGTCTAGAGGTATGATTCTAGATCGGAAGAGCACACGTCTGA426910.37307488387453264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATCAGATCGGAAGAGCAC419050.36620606237291914No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTAGATCGGAAGAGCAC374630.32738760803428396No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCTAGATCGGAAGAGCACAC365550.3194526335769493No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACAGATCGGAAGAGCACA342050.29891608074133635No Hit
GGCTCAATGGTCTAGGGGTATGATTCAAGATCGGAAGAGCACACGTCTGA307420.268653066924431Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTCATAGATCGGAAGAGCACACGTCT306510.2678578216869668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT303470.26520117825631734No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAAGATCGGAAGAGCACACG302550.2643971940601997No Hit
GGCTCAATGGTCTAGGGGTATGATTCCAGATCGGAAGAGCACACGTCTGA297400.2598966303536718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTTGTGTCGATATTTTCTCCTCGGACAGATCGGAAGAGCACACGTCTG291210.2544872149471848Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGATCGGAAGAGCACACG278430.24331882578807273No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGTAGATCGGAAGAGCACACGTC267260.2335574089721665No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACGT265810.23229025996741592No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAAGATCGGAAGAGCACACGTC263950.2306648136578738No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCAAGATCGGAAGAGCACAC249040.21763502630557638No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT242780.21216443818851527No Hit
TACTATTACTTTGAACAAATTAGAGTGCTAGATCGGAAGAGCACACGTCT233000.2036177366254389Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTTGTGTCGATATTTTCTCCTCGGACCAGATCGGAAGAGCACACGTCT228800.19994737399098894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTAGATCGGAAGAGCACACG225120.19673143720651845No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAAGATCGGAAGAGCAC221410.19348928354608766No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAGATCGGAAGAGCACACGTCT217870.19039569218276553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACA215500.18832455898189734No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCAGATCGGAAGAGCAC214280.18725840602617616No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTAGATCGGAAGAGCACACGTC212780.1859475622281583No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA206540.18049445202840406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCAATGGTCTAGGGGTATGATTCTCAAGATCGGAAGAGCACACGTCT205450.17954190553517776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAGATCGGAAGAGCACACGT203750.17805628256409087No Hit
ACAACCTCAACTCATATGGGACTAGATCGGAAGAGCACACGTCTGAACTC198320.17331102801526624Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAGATCGGAAGAGCACACG197420.17252452173645558No Hit
GCATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCAC187200.16359330599262728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAGATCGGAAGAGCACAC184830.1615221727917591No Hit
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGATCGGAAGAGCAC183590.16043854191873103No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCAAGATCGGAAGAGCACAC183370.16024628482835507No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAAGATCGGAAGAGCACA175300.15319394519501903No Hit
TACTATTACTTTGAACAAATTAGAGTGCTTAGATCGGAAGAGCACACGTC167410.14629890681744515No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACA165910.14498806301942732No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATCAGATCGGAAGAGCACA165610.14472589425982374No Hit
ACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGTCTG165160.1443326411204184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCAGATCGGAAGAGCACAC163070.14250619876184686No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT162940.142392592299352No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG157910.13799689609666546No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA157330.13749003649476524No Hit
GGGGATGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG155820.13617045373809392TruSeq Adapter, Index 7 (97% over 36bp)
TAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGTC146540.1280607001076902No Hit
GGCTCAATGGTCTAGGGGTATGATTCTCGAAGATCGGAAGAGCACACGTC144930.12665372776448436No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCT142170.12424177517613152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAAGATCGGAAGAGCACA135470.11838667287831849No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC134020.11711952387356789No Hit
AACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGTCT132800.1160533709178467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCAATGGTCTAGGGGTATGATTCTCAGATCGGAAGAGCACACGTCTG131880.11524938672172909Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTAGATCGGAAGAGCACACGTCTGA125260.10946419609314366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGT122740.10726197851247368No Hit
TGTGGAACTTTCTTGCTAAAATTTTTAAGAAGATCGGAAGAGCACACGTC121950.10657160077885096No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCCAGATCGGAAGAGCACAC121320.10602104638368347No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC120280.10511219468372442No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACTAGATCGGAAGAGCACACGTCT118530.10358287691937026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGTGAGGTCTCCGGACGTGATCACTGTGACGAGATCGGAAGAGCACAC117930.10305853940016312No Hit
TCCGAGGATCGAAACCTTGCTCTGCTACCAAGATCGGAAGAGCACACGTC117630.10279637064055955No Hit
ATGGATGTGATGCCAATGTAATTTGTAACAGATCGGAAGAGCACACGTCT116980.10222833832808514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG116950.10220212145212479No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)