FastQCFastQC Report
Wed 6 Sept 2023
SRR13664943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13664943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14245132
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACAC2965222.081567232932626No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAGATCGGAAGAGCAC2463511.7293697243381108No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAGATCGGAAGAGCACA2404891.6882188245079093No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACGT2313001.6237125777423473No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAGATCGGAAGAGCACACGTC2205791.5484517798782067No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAGATCGGAAGAGCACACGT1851811.2999598740116973No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACGT1706671.198072436254013No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAAGATCGGAAGAGCACACGTC1663271.1676058881026865No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCTG1626011.141449584321156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1609101.1295788624492915TruSeq Adapter, Index 3 (100% over 36bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCAAGATCGGAAGAGCACAC1457731.0233180008440779No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGTC1209150.8488162833450753No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTAGATCGGAAGAGCACA1186890.8331898925190725No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAAGATCGGAAGAGCACAC1184620.8315963656918026No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCTAGATCGGAAGAGCACACGTC1001080.7027523507679676No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAAGATCGGAAGAGCACACGTC969320.6804570150701306No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCTAGATCGGAAGAGCACAC946540.6644655872616695No Hit
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA916480.6433636416987922TruSeq Adapter, Index 3 (100% over 36bp)
CATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACA796740.559306856545801No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAGATCGGAAGAGCACAC781570.5486576045767775No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAGATCGGAAGAGCA778260.5463340037845911No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA663640.46587142892042No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTAGATCGGAAGAGCAC646720.45399368710658494No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC619980.4352223622778644No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAAGATCGGAAGAGCAC613250.4304979413318178No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT611720.429423890210354No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA603050.423337600522059Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAAGATCGGAAGAGCACA552200.3876411956028207No Hit
CTGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGA501980.35238704702771445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTAGATCGGAAGAGCACAC453320.3182280093999831No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACA418140.2935318535482858No Hit
TACTATTACTTTGAACAAATTAGAGTGCTAGATCGGAAGAGCACACGTCT417350.292977278132628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTAGATCGGAAGAGCACACGTCTG397080.2787478557587251Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTGA393680.27636107548880556Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATCAGATCGGAAGAGCAC386300.271180358314686No Hit
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA382750.2686882789152112TruSeq Adapter, Index 3 (100% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAAAGATCGGAAGAGCACACG372120.2612260806007273No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACAGATCGGAAGAGCACACGTCTG365460.25655079924847307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCAC355330.24943959803250682No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCAAGATCGGAAGAGCAC352480.2474389145709566No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACAGATCGGAAGAGCACA351880.24701771805273548No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAGATCGGAAGAGCACACGTCT348050.2443290802780908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA339580.23838318942920292No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACGT337250.23674754295011097No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCAGATCGGAAGAGCA318020.22324819454112463No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG307190.21564559738723377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCAGATCGGAAGAGCAC293500.2060352968298223No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCAAGATCGGAAGAGCACAC266420.18702529397410989No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAAGATCGGAAGAGCACA265930.18668131681756267No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCAAGATCGGAAGAGCAC264070.18537560761107724No Hit
ACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGTCTG263140.18472275300783453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTAGATCGGAAGAGCAC261850.18381718049365917No Hit
TACTATTACTTTGAACAAATTAGAGTGCTTAGATCGGAAGAGCACACGTC261730.18373294119001493No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC259940.182476371577322No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAGATCGGAAGAGCACAC253010.17761155179186827No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG250800.17606014461642056No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGAGATCGGAAGAGCAC249650.1752528512898301No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACG238140.1671728980819553No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAAAGATCGGAAGAGCACA237890.16699739953269652No Hit
TTATAAACGACAATGGATGTGATGCCAATGTAGATCGGAAGAGCACACGT235630.16541089264739703No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGAGATCGGAAGAGCACACG228550.16044077373238802No Hit
TACTATTACTTTGAACAAATTAGAGTGCAAGATCGGAAGAGCACACGTCT228140.1601529561116036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACAC227440.15966156017367897No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAGATCGGAAGAGCACACGT216540.15200982342599564No Hit
AAACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTG202060.1418449474529264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCT201350.1413465315730314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACG200670.14086917551904748No Hit
ATTATTTAGTGAGGTCTCCGGACGTGATAGATCGGAAGAGCACACGTCTG197610.13872107327611988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAGATCGGAAGAGCACACG197140.13839113600351335No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCAAGATCGGAAGAGCA193830.13606753521132692No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAAGATCGGAAGAGCACA190280.13357545581185207No Hit
AACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTGA188190.13210828794004856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATGTGATGCCAATGTAATTTGTAACAAGATCGGAAGAGCACACGTC186880.1311886755419325No Hit
ATGGATGTGATGCCAATGTAATTTGTAACAGATCGGAAGAGCACACGTCT179840.1262466363948049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATCAGATCGGAAGAGCACA176370.12381071653109285No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACAC175330.12308064256617629No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACA175030.12287004430706573No Hit
TAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACACGTC171890.12066578252837531No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGTAGATCGGAAGAGCACACGT171140.12013928688059893No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCT169100.11870721871864717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAAACTGTGCGTCATCGTGTGAACTGCAGGAGATCGGAAGAGCACACG166450.116846934096504No Hit
AGCAAACTGTGCGTCATCGTGTGAACTGCAGGAAGATCGGAAGAGCACAC162150.11382835904925276No Hit
ACAACCTCAACTCATATGGGACTACCCCCTAAGATCGGAAGAGCACACGT159760.1121505929183387No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGGCCAGATCGGAAGAGCACAC158720.11142051895342213No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACCAGATCGGAAGAGCACACGTCT155850.10940579560793119Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCAAGATCGGAAGAGCACACGTC153940.10806498669159402No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAGATCGGAAGAGCACACGTCT152370.1069628558022488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTTGTGTCGATATTTTCTCCTCGGACAAGATCGGAAGAGCACACGTCT149050.10463223506809204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGATCGGAAGAGCACACG148100.10396534058090862No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACG147600.10361434348239104No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCTAAGATCGGAAGAGCACACGT144760.10162067996281116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)