FastQCFastQC Report
Wed 6 Sept 2023
SRR13664944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13664944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20285442
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAGATCGGAAGAGCAC13830196.817790807811829No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAGATCGGAAGAGCACA11576275.706688570059257No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAGATCGGAAGAGCA4382822.160574070804077No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAGATCGGAAGAGCACACGTC3904871.9249617533598724No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC3028011.4927010217475172TruSeq Adapter, Index 6 (100% over 36bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAAGATCGGAAGAGCACAC2985671.471828910604955No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAGATCGGAAGAGCACACGT2535181.249753394577254No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAAGATCGGAAGAGCACACGTC2443421.2045189845998918No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAAGATCGGAAGAGCAC2350361.1586437209502263No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACGT2228581.098610520786286No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCTAGATCGGAAGAGCACACGTC2062611.0167932254076595No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGTC1973850.9730377085202284No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCAGATCGGAAGAGCAC1899440.9363562302463017No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTAGATCGGAAGAGCACA1873180.9234109860657708No Hit
ATAATTTTGAACGAAAGGGAATACGGTTAGATCGGAAGAGCACACGTCTG1835120.9046487623981769Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCTG1513210.7459586042049269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAACAGATCGGAAGAGCACA1476190.7277090634751759No Hit
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1462580.7209998184905214TruSeq Adapter, Index 6 (100% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAGATCGGAAGAGCACAC1446870.713255348342915No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACGT1255490.6189118284925712No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAAGATCGGAAGAGCACACGTC1181490.5824324656076018No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAGATCGGAAGAGCACACGT1130660.5573750870205343No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTAGATCGGAAGAGCACAC1083570.5341613951522476No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1045810.515547060793647No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT984660.4854022899772162No Hit
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC923750.4553758306079798TruSeq Adapter, Index 6 (100% over 36bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAAAGATCGGAAGAGCACA888260.4378805253540939No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAGATCGGAAGAGCACACG840260.41421823591519474No Hit
GGCTCAATGGTCTAGGGGTATGATTCTAGATCGGAAGAGCACACGTCTGA816570.4025399101483714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA726440.35810903208320527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA688720.3395144162991371No Hit
ACAACCTCAACTCATATGGGACTAGATCGGAAGAGCACACGTCTGAACTC675240.3328692566817129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCAGATCGGAAGAGCA643980.3174591906846299No Hit
GCATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCAC637230.3141316812322847No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCAGATCGGAAGAGCA634530.31280067745134665No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAGAGATCGGAAGAGCACA634530.31280067745134665No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGAGATCGGAAGAGCAC627650.30940908263177114No Hit
ACAACCTCAACTCATATGGGACTACCCCCTAAGATCGGAAGAGCACACGT601780.2966560945529311No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAAGATCGGAAGAGCACACG598530.2950539603721723No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAGATCGGAAGAGCACACGTCT579630.28573693390560584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAAGATCGGAAGAGC560220.276168495613751No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACAC556750.2744579092730639No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAAGATCGGAAGAGCA536480.26446552162876213No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAGATCGGAAGAGCACACGTCT520910.25679006649201924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAGTAGATCGGAAGAGCACA504660.2487793955882253No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACAGATCGGAAGAGCACACGTCTG503310.24811389369775627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGA483780.23848629968230417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG481050.2371405069704668No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG477390.23533625740075076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTAAGATCGGAAGAGCACACGTCTG455750.22466850857871373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAGTAGATCGGAAGAGCAC454610.22410652920453988No Hit
ATAATTTTGAACGAAAGGGAATACGGTAGATCGGAAGAGCACACGTCTGA441020.21740714350715157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTGA370070.1824313219302789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCAGATCGGAAGAGCAC369670.1822341361849547No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAGATCGGAAGAGCACAC334150.16472404200016938No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCAAGATCGGAAGAGCACAC333480.1643937558767514No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCAAGATCGGAAGAGCA318880.1571964761724196No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTAGATCGGAAGAGCAC305750.15072385408215408No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGAGATCGGAAGAGCAC302930.14933369457761878No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCTAGATCGGAAGAGCACAC300730.1482491729783359No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAAGATCGGAAGAGCACA297480.1466470387975771No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAAGATCGGAAGAGCACA295920.14587801439081288No Hit
ATAATTTTGAACGAAAGGGAATACGGTCAGATCGGAAGAGCACACGTCTG290340.14312727324354088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAACTAGATCGGAAGAGCAC276870.1364870432697498No Hit
GGCTCAATGGTCTAGGGGTATGATTCAAGATCGGAAGAGCACACGTCTGA267090.1316658517965741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTAGATCGGAAGAGCACACGTC262410.12935877857628145No Hit
ATTATTTAGTGAGGTCTCCGGACGTGATAGATCGGAAGAGCACACGTCTG262100.12920595962365522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTGA260140.12823974947156686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCTAGATCGGAAGAGCA259590.12796861907174611No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATCAGATCGGAAGAGCACA254250.1253361893716686No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGAGATCGGAAGAGCACACG254140.12528196329170446No Hit
TCCCATATTGTCTAGTGGTTAGGATATCCGGCTAGATCGGAAGAGCACAC253690.12506012932821478No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACG243350.11996287781158527No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCAAGATCGGAAGAGCAC243110.11984456636439078No Hit
AAACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTG243080.11982977743349146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACAAGATCGGAAGAGCACACGTCTGAACTC242880.11973118456082939Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTTGTGTCGATATTTTCTCCTCGGACCAGATCGGAAGAGCACACGTCT237920.11728608131880983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCGAGATCGGAAGAGCACACGT228430.11260784951099413No Hit
CATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACA226420.11161699114074024No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAATAGATCGGAAGAGCAC221610.10924583255321724No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT212730.1048683090070209No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGT204660.10089008659510598No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGTAGATCGGAAGAGCACACGT204390.10075698621701218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)