FastQCFastQC Report
Wed 6 Sept 2023
SRR13664945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13664945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11926375
Total Bases906.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAGATCGGAAGAGCAC5829864.8882078586326525No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA5012704.203037385626395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATAGATCGGAAGAGCACA5005124.19668172432948No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACGT2089401.7519153975956652No Hit
AACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTGA1772141.4858999486432383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAGATCGGAAGAGCA1615831.3548374925323075No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGTC1567351.3141880915198456No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAGATCGGAAGAGCACACGTC1449661.215507645868925No Hit
AACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTGA1362131.142115688966681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGAA1348431.1306285438785884Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAAGATCGGAAGAGCACAC1330661.1157287943738143No Hit
TGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGAA940490.7885799331314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACGT859010.7202607665782772No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCAGATCGGAAGAGCAC771010.6464747251365146No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAGATCGGAAGAGCACACGT746680.6260745616333546No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATAAGATCGGAAGAGCAC746290.6257475553133287No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCTG727010.6095817044156334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTAGATCGGAAGAGCACACGTCTG715980.6003332949031035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAGATCGGAAGAGCACACGT702580.5890976931381078No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAAGATCGGAAGAGCACACGTC670880.5625179486642001No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA655230.5493957719759776TruSeq Adapter, Index 5 (100% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCAAGATCGGAAGAGCACACGTC640280.5368605297083145No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG623840.5230759556026034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAACAGATCGGAAGAGCACA606250.5083271320916875No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCTAGATCGGAAGAGCACACGTC595920.49966565699971704No Hit
CTGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGA529190.44371403716552593Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACA519590.43566465082642464No Hit
AACTCTAAGCGGTGGATCACTCGGCAAGATCGGAAGAGCACACGTCTGAA465190.39005146157151693Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTATTTAGTGAGGTCTCCGGACGTGATAGATCGGAAGAGCACACGTCTG459820.38554883608808205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTAGATCGGAAGAGCACA459460.3852469841003658No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACAC449530.3769209001058578No Hit
AAACTGTGCGTCATCGTGTGAACTGCAGATCGGAAGAGCACACGTCTGAA424010.3555229480877467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTAAGCGGTGGATCACTCGGCTCATAGATCGGAAGAGCACACGTCT407110.3413526742199537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAGATCGGAAGAGCACAC365440.30641330664179184No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACGT352480.295546635084005No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAGATCGGAAGAGCACACG339840.2849482764041882No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCAC322730.27060192221022733No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACG312430.2619656014505665No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA310500.26034733940530963No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAAAGATCGGAAGAGCACA302950.25401683244070394No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACAGATCGGAAGAGCACACGTCTG302740.25384075211453605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTATTACTTTGAACAAATTAGAGTAGATCGGAAGAGCACACGTCTGAA294720.24711616061041178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTAAGCGGTGGATCACTCGGCCAGATCGGAAGAGCACACGTCTGAA291510.2444246470532748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC288860.24220268103258535No Hit
AAACTCTAAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA284960.2389326178323254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTAAGATCGGAAGAGCACACGT281080.23567932418693863No Hit
TGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAA233450.19574262925658464Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGAGATCGGAAGAGCAC232770.1951724643908983No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAGAGATCGGAAGAGCACA225830.18935342884992296No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCAGATCGGAAGAGCA225370.18896772908784104No Hit
AAACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTG220570.18494303591829034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA218630.18331638909559694TruSeq Adapter, Index 5 (100% over 36bp)
ACAACCTCAACTCATATGGGACTAGATCGGAAGAGCACACGTCTGAACTC218620.18330800431816038Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAATTTTGAACGAAAGGGAATACGGTTCAGATCGGAAGAGCACACGTCT199700.16744400540818144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACAC197980.16600182368909244No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACG197920.16595151502447308No Hit
TACTATTACTTTGAACAAATTAGAGTGCTAGATCGGAAGAGCACACGTCT184550.1547410675917871Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTAGATCGGAAGAGCACACGTCTGA180530.15137038706228842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCAAGATCGGAAGAGCACAC169140.1418201255620421No Hit
TGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACGTC168500.14128349980610203No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCTAGATCGGAAGAGCACAC167600.14052886983681126No Hit
AAACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTG166340.13947238787980423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGT164730.13812243871251742No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCT159690.13389651088448923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGAGATCGGAAGAGCAC159080.1333850394608588No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGAGATCGGAAGAGCACACG156070.13086122145245307No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAAGATCGGAAGAGCA154070.1291842659651403No Hit
CATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACA154070.1291842659651403No Hit
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA153160.12842125121841297TruSeq Adapter, Index 5 (100% over 36bp)
TGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACACG148810.12477387303350766No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAAGATCGGAAGAGCACACG146470.12281183511335171No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAAGATCGGAAGAGC146210.12259383090000105No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACAC143280.12013709111108782No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGGAAGAGCACACGTCTGAAC135170.11333703661003448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATAGATCGGAAGAGCACAC134730.11296810640282566No Hit
AACTCTAAGCGGTGGATCACTCGGCGAGATCGGAAGAGCACACGTCTGAA132690.11125761180576664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCT129970.10897695234302124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCAGATCGGAAGAGCA129590.10865833080043182No Hit
ATAATTTTGAACGAAAGGGAATACGGTAAGATCGGAAGAGCACACGTCTG129510.10859125258093931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCAGATCGGAAGAGCACACGTCTGAAC127390.10681367976438776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGTGCGTCATCGTGTGAACTGTAGATCGGAAGAGCACACGTCTGAA122650.10283929525945645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)