FastQCFastQC Report
Thu 14 Sept 2023
SRR13664946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13664946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160289139
Total Bases12.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAGATCGGAAGAGCAC59852823.7340533721377094No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA54587353.4055551324659623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATAGATCGGAAGAGCACA51081563.186838504385503No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACGT22542311.4063529282542344No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAGATCGGAAGAGCACACGTC20544981.2817449846056008No Hit
AACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTGA19849561.2383596370805885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGTC16952051.057591930792017No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTAGATCGGAAGAGCA16494641.0290553747375235No Hit
AACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTGA15439630.9632361928152848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTAAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGAA14568270.9088744309743905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAAGATCGGAAGAGCACAC13475940.8407269565531823No Hit
TGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGAA12410470.7742552039037405Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAAGATCGGAAGAGCACACGT10615340.6622619639874664No Hit
ATAATTTTGAACGAAAGGGAATACGGTTAGATCGGAAGAGCACACGTCTG10122650.6315243854419856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTAAGCGGTGGATCACTCGGCTCATGAAGATCGGAAGAGCACACGT9335120.5823925475075389No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCAAGATCGGAAGAGCACACGTC9058600.5651412227000608No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA8590920.5359639494975389TruSeq Adapter, Index 5 (100% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCTAGATCGGAAGAGCACACGTC8404400.524327477983396No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG8156380.5088541900521407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATAAGATCGGAAGAGCAC7859680.4903438903617793No Hit
AACTCTAAGCGGTGGATCACTCGGCTCAAGATCGGAAGAGCACACGTCTG7791450.4860872076928431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATCAGATCGGAAGAGCAC7689270.4797124775871433No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACA7387960.4609145726336455No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAAGATCGGAAGAGCACACGTC7300350.45544882488887783No Hit
CTGTTGTGTCGATATTTTCTCCTCGGAAGATCGGAAGAGCACACGTCTGA7127210.4446470948976774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAGATCGGAAGAGCACACGT6985370.4357980861073812No Hit
ATTATTTAGTGAGGTCTCCGGACGTGATAGATCGGAAGAGCACACGTCTG6827920.42597521220698553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCAGATCGGAAGAGCACAC6707660.4184725204619135No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTAGATCGGAAGAGCACA6456030.40277401452633665No Hit
AAACTGTGCGTCATCGTGTGAACTGCAGATCGGAAGAGCACACGTCTGAA6040350.37684087878218625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAACAGATCGGAAGAGCACA5975810.3728144050982768No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAATAGATCGGAAGAGCACAC5158440.3218209313607954No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACGT5035050.314122967495633No Hit
AACTCTAAGCGGTGGATCACTCGGCAAGATCGGAAGAGCACACGTCTGAA5033330.3140156614104715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCAC4748040.2962172003431873No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACG4481520.2795897481238576No Hit
TACTATTACTTTGAACAAATTAGAGTAGATCGGAAGAGCACACGTCTGAA4462130.27838005917543795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA4383810.2734938890650601No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATAGATCGGAAGAGCACACGTCT4339650.270738867715797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC4212450.26280320839455007No Hit
CTGTTGTGTCGATATTTTCTCCTCGGACAGATCGGAAGAGCACACGTCTG4054870.2529722241505084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTCTAAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA3814100.2379512438456607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAA3407790.21260267671660527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAAGATCGGAAGAGCACACG3372450.21039791099008898No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAAAGATCGGAAGAGCACA3243270.20233872489638866No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACAC2968630.18520468813548246No Hit
AACTCTAAGCGGTGGATCACTCGGCCAGATCGGAAGAGCACACGTCTGAA2945600.18376790956497682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTCTAAGCGGTGGATCACTCGGCTAAGATCGGAAGAGCACACGTCTG2936480.18319893776458554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA2896300.1806922177054055TruSeq Adapter, Index 5 (100% over 36bp)
ATAATTTTGAACGAAAGGGAATACGGTTCAGATCGGAAGAGCACACGTCT2872570.17921176805372946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTAAGATCGGAAGAGCACACGT2830850.17660897161597455No Hit
TACTATTACTTTGAACAAATTAGAGTGCTAGATCGGAAGAGCACACGTCT2813450.17552343331259643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTCTAAGCGGTGGATCACTCGGCTCATGGAGATCGGAAGAGCACACG2615500.16317387543019993No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCAAGATCGGAAGAGCACAC2601570.16230482091490928No Hit
ATAATTTTGAACGAAAGGGAATACGGTAGATCGGAAGAGCACACGTCTGA2548780.15901139752207416Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCTAGATCGGAAGAGCACAC2526870.15764449268144112No Hit
ACAACCTCAACTCATATGGGACTAGATCGGAAGAGCACACGTCTGAACTC2419560.15094971593802123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGAGATCGGAAGAGCAC2401730.14983735111335272No Hit
TGGTTGAACTCTAGATAACATGCAGATCGTAGATCGGAAGAGCACACGTC2389810.1490936949882799No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAAGAGATCGGAAGAGCACA2341840.14610097818293227No Hit
AAACTCTAAGCGGTGGATCACTCGGCTTAGATCGGAAGAGCACACGTCTG2314770.1444121550868147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCT2296880.14329604702661733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGTATGGCTTCTAAACCATTTAAAGTTTATAAGATCGGAAGAGCACA2288150.14275140625716382No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCAGATCGGAAGAGCA2245530.14009246128647557No Hit
AAACTCTAAGCGGTGGATCACTCGGCTCATGAGATCGGAAGAGCACACGT2206670.13766809240893108No Hit
ATAATTTTGAACGAAAGGGAATACGGTTCCAAAGATCGGAAGAGCACACG2056800.1283181139303518No Hit
TGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACACG2052800.1280685648950925No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTATAGATCGGAAGAGCACAC2007490.12524179819819234No Hit
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA2001340.12485811655648109TruSeq Adapter, Index 5 (100% over 36bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATAGATCGGAAGAGCACAC1979110.12347124779302733No Hit
ATAATTTTGAACGAAAGGGAATACGGTAAGATCGGAAGAGCACACGTCTG1880210.11730114789624019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGGAAGAGCACACGTCTGAAC1873760.11689875007688451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCAGATCGGAAGAGCA1790830.11172497470337026No Hit
AAAACTCTAAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCT1761500.10989515640233118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTATTACTTTGAACAAATTAGAGTGCTTAGATCGGAAGAGCACACGTC1758000.10967680099647924No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGA1747690.10903358835809829Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGTGCGTCATCGTGTGAACTGTAGATCGGAAGAGCACACGTCTGAA1736860.10835793434513365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAAACTGTGCGTCATCGTGTGAACTGCAGGAGATCGGAAGAGCACACG1704670.10634968848388412No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGAGATCGGAAGAGCAC1648300.10283291870449189No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGAGATCGGAAGAGCACACG1638780.10223899200057465No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTAAGATCGGAAGAGCA1607830.10030810634025554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)