FastQCFastQC Report
Wed 6 Sept 2023
SRR13679861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13679861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804004
Total Bases672.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAA1513660.9007734109084955No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC705690.4199534825152386No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC446840.2659128145887135No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGT410430.2442453596178625No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT393200.23399185098979983No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTAT377010.22435724247625746No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTA370770.2206438417891355No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC363510.21632344291277247No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGG352580.2098190407476694No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCT342640.20390378388388863No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA335840.19985712928894803No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCG319590.19018681499956797No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG315410.18769931261620743No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG311430.18533082948563925No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT301260.17927870048114722No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG300810.1790109071623644No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTG288830.17188165392010143No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC287960.17136392017045463No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCC287810.17127465573086034No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT280690.16703757033145195No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT275260.1638061976181391No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGA275230.16378834473022025No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTG273180.16256839738909845No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA269280.16024752195964725No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC254660.15154721458052497No Hit
CCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACC251870.1498868960040714No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCG251000.14936916225442462No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG237140.14112112803591334No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCT235010.1398535729936746No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATC232190.1381754015293022No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTC230280.13703876766513504No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAAT227820.1355748308557889No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA226240.13463457875872917No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT218160.12982620094591743No Hit
GTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTC217380.12936202586002718No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGC214860.1278623832748433No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA213290.1269280821404232No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA213110.12682096481291008No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTA209950.12494046061879063No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG204430.1216555292417212No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT203040.12082834543481423No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCT201380.11984048563663754No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATT196940.11719825822464694No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC191870.11418112016636035No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGAC188690.11228871404696165No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAAC187320.11147343216533392No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGC182220.10843844121912849No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAG181590.10806353057283252No Hit
GTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG181090.1077659824408516No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCC180730.10755174778582534No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC180630.10749223815942914No Hit
GTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGT177280.10549866567515695No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC176650.10512375502886098No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGAT176310.10492142229911396No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT170920.10171385343635958No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC168530.10029157336549074No Hit
CTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTA168490.10026776951493227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)