FastQCFastQC Report
Wed 6 Sept 2023
SRR13679862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13679862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15251994
Total Bases610 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAA1420650.9314519793280801No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC606030.39734476685474696No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC549470.36026109110716936No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG522540.3426043834006229No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTAT487220.3194467556176589No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT428330.28083541076661844No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTA397430.26057576471640365No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTA384710.2522358715850531No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA370260.24276170053568077No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC367820.24116190971488713No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCG358500.23505123330103592No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG355280.2329400339391689No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGG354380.23234994716100726No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCC333020.21834522095930542No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGT328050.2150866306399019No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA316940.20780233718948488No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATC309550.20295706908880243No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC293140.19219782016698933No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCT291650.19122089872314402No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGA290170.19025053379905604No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT283670.18598879595677784No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG282010.1849004136770576No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCG268290.1759048685699719No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTG266520.17474436457292075No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT259980.17045640065161316No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTC255930.167801010149886No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT255520.1675321928398346No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA253480.16619466280933495No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGC249460.16355894186687983No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC248470.1629098464109021No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGG239040.15672704827972No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATT238570.1564188918511245No Hit
CCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACC238160.1561500745410731No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC237900.15597960502738198No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA236210.15487155318838966No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCT231990.1521047018507875No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT231680.15190144973830963No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG230040.15082618049810403No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC228730.14996727640989105No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGAC223910.14680703388684782No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG221900.1454891734156203No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGC220760.14474173016328226No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC211960.13897199277681332No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGC211510.13867694938773253No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAA208780.13688701949397566No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCG206250.13522821999536586No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT200200.13126152554216844No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCC197410.1294322565298675No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCT194220.1273407267272725No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA185460.12159721541983297No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC184970.12127594595172278No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTC182610.11972860728898792No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC180690.1184697554955765No Hit
GGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACT178220.11685029511551079No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTC177860.11661426040424616No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA173180.11354580915780585No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC171440.11240497472002678No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGG171130.11220172260754889No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG166030.10885789753129983No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA164850.10808422819993241No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAA164520.10786786304793983No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT159980.10489120307810243No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA159760.10474695964344072No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT159490.10456993360999224No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC159160.10435356845799966No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTG156650.10270788199890453No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGG156570.10265542984084573No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATC155210.10176374315384598No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAG154850.10152770844258135No Hit
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGG153380.10056390003825075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)