FastQCFastQC Report
Wed 6 Sept 2023
SRR13679863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13679863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15986948
Total Bases639.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAA1813361.134275285063791No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC664860.4158767514600035No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG636280.3979996682293581No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTAT630050.39410273930959183No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC565400.3536635010009415No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT551180.34476874510381844No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTA513450.32116824299422253No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA500350.3129740585882934No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG488160.3053490885189593No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC480400.30049512890140134No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTA445310.27854597387819113No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCG440260.27538714706521844No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGG437210.27347934077223496No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCC417600.26121308457374104No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA382930.23952664385972858No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGA380610.23807546005654112No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGG335160.209646018739787No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT326950.2045105795052314No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGT326680.20434169173503286No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTC321920.2013642628974586No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCT313320.1959848746615051No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC312910.19572841545490732No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATC307960.19263213966793413No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA305540.1911184048387472No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGC302740.18936697611076234No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGAC301370.18851002705456976No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCG300610.1880346392569739No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT294090.18395631236180915No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC292520.18297426125361763No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG284180.17775750568526277No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC284060.17768244445406342No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATT273260.17092693364612183No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA270970.16949451515073422No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG262520.16420895345377992No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT260210.1627640247531924No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAA258750.1618507797736003No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCG258080.16143168789940393No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTG252760.15810397331623272No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCT247600.15487634037466064No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA244410.1528809626452779No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT244310.15281841161927842No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTC241000.15074797265869633No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAA238760.14934682967630847No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG233450.14602537019573716No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC232890.1456750844501402No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA223810.13999545128938934No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGC223460.13977652269839122No Hit
GGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACT222120.13893833894999846No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC218620.1367490530400174No Hit
GTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCG214700.13429705282083862No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCT210840.13188258321725949No Hit
CCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACC209350.13095057292986753No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC208760.13058152187647074No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAG206560.12920539930448263No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC205420.1284923176080888No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGG204590.12797314409229327No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGC204300.12779174611689487No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT202160.12645315416050643No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA200400.12535225610291595No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTC200380.12533974589771607No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA197740.12368839881133034No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAAC195920.12254997013814019No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA195140.12206207213534441No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCA186090.11640120428239337No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG186050.11637618387199358No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGC185120.1157944593301986No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT184160.1151939694806038No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT182500.11415562244901278No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCC179140.11205390797543095No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG177960.11131580586863735No Hit
CCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC177460.11100305073864004No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAG174780.10932668324185454No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCC172840.10811319333746502No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTC171220.10709986671627379No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGG170180.10644933604587942No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT169860.10624917276268114No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTG168790.10557987678448694No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAG168330.10529214206488943No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCG167920.10503568285829167No Hit
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGG166270.10400359092930059No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC163360.10218335607271632No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC162670.10175175399332005No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTG161220.1008447641163279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)