FastQCFastQC Report
Wed 6 Sept 2023
SRR13723996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13723996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59977359
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length48
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA12110812.019230289883221No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA10192581.6994046036605246No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA8526501.4216197815579041No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA7475831.2464420115597288No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAA6655871.1097304234419525No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA4610630.7687284129999788No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA4015500.6695026368200041No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA3685740.6145218898351292No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA3430520.571969165898085No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAA3073940.5125167315219731No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAA2905750.48447448311286934No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAA2851690.4754610819059239No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA2780940.46366496397415563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA2563860.42747130629743135No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAAAAAAAAAAAAAAAA2409620.40175493555826625No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA2368080.39482898871889305No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2107140.35132257157238284No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAA2101190.3503305305590398No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAA2037800.33976154235133965No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAA1986700.3312416607073346No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAA1966330.3278453791204778No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAA1838650.3065573460812104No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAAAAAAAA1698490.28318852785765375No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAA1681120.28029243501702034No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAAAAAAAAAAAAAAAAAA1589980.2650967009067538No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1586140.2644564593115879No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAAA1543820.2574004633981966No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAA1468090.2447740321476976No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAA1288720.21486774701100128No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAT1189430.1983131668068279No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAA1174530.1958288960339184No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAAAAAAAAAAAAAAAA1164570.19416826939645676No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAAAAAAAAAAAAAA1115180.18593349533779904No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAAAAAAAAA1109190.18493478514117304No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAAAAAAAAAAAAAAAA1105500.18431955298331826No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAAAAAAAAAAAAAAAAA1104350.18412781396393263No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1079630.1800062586950519No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1060920.17688674821443873No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1033890.17238004761096598No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAA991280.16527570011877316No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGAAAAAAAAAAAAAAAAAAA988130.16475050193523857No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAA974560.16248798150648813No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAAAAAAAAAAA971440.16196778521041583No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAA970300.16177771348685094No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA963750.1606856347242632No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAAAAAAAAAAAAAAA942770.15718764809234098No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAA900420.1501266502914875No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAAAAAAAAAAAAAAAAA890180.148419339371045No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAT869050.14489634330181161No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGAAAAAAAAAAAAAAAAAA841150.14024458796193412No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAAAA839110.13990445961450218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827970.13804709207019267No Hit
ATCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAA822790.13718343283504697No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAA762280.12709462582372125No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAA736370.12277466235217194No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAA730360.12177261756390441No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAA706010.11771275224039125No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAAAAAAAAAAAAAAA703910.11736262011803487No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699900.11669403449391627No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAAA691040.11521681039673654No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAAT689190.11490836066989879No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAA685560.11430313228696848No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAA685550.11430146499114774No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAAAAAAAAAAAAAAA677960.11303598746320256No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAA677310.11292761323485416No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAAAAAAAAAAAAAAAAA661020.11021158834286118No Hit
ACAAGACACGTGCTGAAGTCAAAAAAAAAAAAAAAAAAAATCGTATGC648000.10804076918425166No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATC642950.10719878479477564No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAAAAAAAAAAAAAAAAA639690.1066552463572129No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAA633290.10558817703193632No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA623500.10395589442342736No Hit
AAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAA608420.10144161232574446No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTCCAAAAAAAAAAAAAAAAAAA607750.10132990350575456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)